Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid- Ordered and Liquid-Disordered Phases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00431198" target="_blank" >RIV/61388963:_____/14:00431198 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388955:_____/14:00431198

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bpj.2014.07.018" target="_blank" >http://dx.doi.org/10.1016/j.bpj.2014.07.018</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bpj.2014.07.018" target="_blank" >10.1016/j.bpj.2014.07.018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid- Ordered and Liquid-Disordered Phases

Popis výsledku v původním jazyce

Cholesterol is important for the formation of microdomains in supported lipid bilayers and is enriched in the liquidordered phase. To understand the interactions leading to this enrichment, we developed an AFM-based single-lipid-extraction (SLX) approachthat enables us to determine the anchoring strength of cholesterol in the two phases of a phase-separated lipid membrane. As expected, the forces necessary for extracting a single cholesterol molecule from liquid-ordered phases are significantly higherthan for extracting it from the liquid-disordered phases. Interestingly, application of the Bell model shows two energy barriers that correlate with the head and full length of the cholesterol molecule. The resulting lifetimes for complete extraction are90 s and 11 s in the liquid-ordered and liquid-disordered phases, respectively. Molecular dynamics simulations of the very same experiment show similar force profiles and indicate that the stabilization of cholesterol in the liquid-order

Název v anglickém jazyce

Single Lipid Extraction: The Anchoring Strength of Cholesterol in Liquid- Ordered and Liquid-Disordered Phases

Popis výsledku anglicky

Cholesterol is important for the formation of microdomains in supported lipid bilayers and is enriched in the liquidordered phase. To understand the interactions leading to this enrichment, we developed an AFM-based single-lipid-extraction (SLX) approachthat enables us to determine the anchoring strength of cholesterol in the two phases of a phase-separated lipid membrane. As expected, the forces necessary for extracting a single cholesterol molecule from liquid-ordered phases are significantly higherthan for extracting it from the liquid-disordered phases. Interestingly, application of the Bell model shows two energy barriers that correlate with the head and full length of the cholesterol molecule. The resulting lifetimes for complete extraction are90 s and 11 s in the liquid-ordered and liquid-disordered phases, respectively. Molecular dynamics simulations of the very same experiment show similar force profiles and indicate that the stabilization of cholesterol in the liquid-order

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP208%2F12%2FG016" target="_blank" >GBP208/12/G016: Řízení struktury a funkce biomolekul na molekulové úrovni: souhra teorie a experimentu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biophysical Journal

ISSN

0006-3495

e-ISSN

—

Svazek periodika

107

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1167-1175

Kód UT WoS článku

000341275100017

EID výsledku v databázi Scopus

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