Development and characterization of microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitinae) using pyrosequencing technology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00444428" target="_blank" >RIV/61388963:_____/15:00444428 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12686-014-0411-5" target="_blank" >http://dx.doi.org/10.1007/s12686-014-0411-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12686-014-0411-5" target="_blank" >10.1007/s12686-014-0411-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development and characterization of microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitinae) using pyrosequencing technology

Popis výsledku v původním jazyce

Eighteen microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitidae: Termitinae) were developed using a procedure of microsatellite-enriched libraries pyrosequencing. They were optimized in four multiplex PCR sets, andtested on 38 individuals collected in French Guiana. The number of alleles per locus ranged from 3 to 13. The expected and observed heterozygosities varied from 0.279 to 0.867 and from 0.237 to 0.789, respectively. Cross-species amplifications of these loci were performed in eight other neotropical species of Termitidae. These new microsatellite markers will constitute useful tools to study population genetics, reproductive strategies and dispersal patterns of C. tuberosus, which is a common representative of the humivorous guild, fulfilling an essential function in soil nutrient recycling.

Název v anglickém jazyce

Development and characterization of microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitinae) using pyrosequencing technology

Popis výsledku anglicky

Eighteen microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitidae: Termitinae) were developed using a procedure of microsatellite-enriched libraries pyrosequencing. They were optimized in four multiplex PCR sets, andtested on 38 individuals collected in French Guiana. The number of alleles per locus ranged from 3 to 13. The expected and observed heterozygosities varied from 0.279 to 0.867 and from 0.237 to 0.789, respectively. Cross-species amplifications of these loci were performed in eight other neotropical species of Termitidae. These new microsatellite markers will constitute useful tools to study population genetics, reproductive strategies and dispersal patterns of C. tuberosus, which is a common representative of the humivorous guild, fulfilling an essential function in soil nutrient recycling.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Conservation genetics resources

ISSN

1877-7252

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

521-524

Kód UT WoS článku

000354213300052

EID výsledku v databázi Scopus

2-s2.0-84928981810