Synthesis of Derivatized Chitooligomers using Transglycosidases Engineered from the Fungal GH20 beta-N-Acetylhexosaminidase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00445811" target="_blank" >RIV/61388963:_____/15:00445811 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/15:00445811 RIV/61388971:_____/15:00445811

Výsledek na webu

<a href="http://dx.doi.org/10.1002/adsc.201500075" target="_blank" >http://dx.doi.org/10.1002/adsc.201500075</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/adsc.201500075" target="_blank" >10.1002/adsc.201500075</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Synthesis of Derivatized Chitooligomers using Transglycosidases Engineered from the Fungal GH20 beta-N-Acetylhexosaminidase

Popis výsledku v původním jazyce

The synthesis of oligosaccharides using mutant glycosidases has been dynamically developing due to the need for novel carbohydrate-based materials. Chitooligomers (beta-1 -> 4-linked oligomers of N-acetylglucosamine) are bioactive compounds applicable inmany industrial and pharmacological areas; however, their accessibility is still rather low. In this work, GH20 beta-N-acetylhexosaminidase from the fungus Talaromyces flavus was engineered by site-directed mutagenesis to obtain three efficiently transglycosylating variants with ca. 200-times suppressed hydrolytic activity. Thus, we have prepared the first GH20 transglycosidases. In the reactions catalyzed by these mutant beta-N-acetylhexosaminidases we were able to easily prepare and isolate both natural and modified chitooligomers in sufficient amounts for their complete spectral characterization and possible further application. The presented method for the synthesis of chitooligomers with aglycones suitable for linking to other bio

Název v anglickém jazyce

Synthesis of Derivatized Chitooligomers using Transglycosidases Engineered from the Fungal GH20 beta-N-Acetylhexosaminidase

Popis výsledku anglicky

The synthesis of oligosaccharides using mutant glycosidases has been dynamically developing due to the need for novel carbohydrate-based materials. Chitooligomers (beta-1 -> 4-linked oligomers of N-acetylglucosamine) are bioactive compounds applicable inmany industrial and pharmacological areas; however, their accessibility is still rather low. In this work, GH20 beta-N-acetylhexosaminidase from the fungus Talaromyces flavus was engineered by site-directed mutagenesis to obtain three efficiently transglycosylating variants with ca. 200-times suppressed hydrolytic activity. Thus, we have prepared the first GH20 transglycosidases. In the reactions catalyzed by these mutant beta-N-acetylhexosaminidases we were able to easily prepare and isolate both natural and modified chitooligomers in sufficient amounts for their complete spectral characterization and possible further application. The presented method for the synthesis of chitooligomers with aglycones suitable for linking to other bio

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GP13-06818P" target="_blank" >GP13-06818P: Nové syntetické přístupy s využitím mutantní a přirozené beta-N-acetylhexosaminidasy z Talaromyces flavus</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced Synthesis & Catalysis

ISSN

1615-4150

e-ISSN

—

Svazek periodika

357

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

1941-1950

Kód UT WoS článku

000355235700033

EID výsledku v databázi Scopus

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