The effects of smoke derivatives on in vitro seed germination and development of the leopard orchid Ansellia africana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00458880" target="_blank" >RIV/61388963:_____/16:00458880 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/plb.12374" target="_blank" >http://dx.doi.org/10.1111/plb.12374</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/plb.12374" target="_blank" >10.1111/plb.12374</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effects of smoke derivatives on in vitro seed germination and development of the leopard orchid Ansellia africana

Popis výsledku v původním jazyce

Plant-derived smoke and smoke-isolated compounds stimulate germination in seeds from over 80 genera. It has also been reported that smoke affects overall plant vigour and has a stimulatory effect on pollen growth. The effect of smoke on orchid seeds, however, has not been assessed. In South Africa, orchid seeds from several genera may be exposed to smoke when they are released from their seedpods. It is therefore possible that smoke may affect their germination and growth. Therefore, the effects of smoke [applied as smoke-water (SW)] and two smoke-derived compounds, karrikinolide (KAR(1)) and trimethylbutenolide (TMB), were investigated on the germination and growth of orchid seeds in vitro. The effect of SW, KAR(1) and TMB were investigated on the endangered epiphytic orchid, Ansellia africana, which is indigenous to tropical areas of Africa. Smoke-water, KAR(1) and TMB were infused in half-strength MS medium. The number of germinated seeds and number of seeds and protocorm bodies to reach predetermined developmental stages were recorded on a weekly basis using a dissecting microscope for a 13-week period. Infusing SW 1: 250 (v:v) into half-strength MS medium significantly increased the germination rate index (GRI) and the development rate index (DRI) of the A. africana seeds. All the SW treatments significantly increased the number of large protocorm bodies at the final stage of development. Infusing KAR(1) into the growing medium had no significant effect on germination or development of the seeds. The TMB treatment, however, significantly reduced the GRI and DRI of A. africana seeds.

Název v anglickém jazyce

The effects of smoke derivatives on in vitro seed germination and development of the leopard orchid Ansellia africana

Popis výsledku anglicky

Plant-derived smoke and smoke-isolated compounds stimulate germination in seeds from over 80 genera. It has also been reported that smoke affects overall plant vigour and has a stimulatory effect on pollen growth. The effect of smoke on orchid seeds, however, has not been assessed. In South Africa, orchid seeds from several genera may be exposed to smoke when they are released from their seedpods. It is therefore possible that smoke may affect their germination and growth. Therefore, the effects of smoke [applied as smoke-water (SW)] and two smoke-derived compounds, karrikinolide (KAR(1)) and trimethylbutenolide (TMB), were investigated on the germination and growth of orchid seeds in vitro. The effect of SW, KAR(1) and TMB were investigated on the endangered epiphytic orchid, Ansellia africana, which is indigenous to tropical areas of Africa. Smoke-water, KAR(1) and TMB were infused in half-strength MS medium. The number of germinated seeds and number of seeds and protocorm bodies to reach predetermined developmental stages were recorded on a weekly basis using a dissecting microscope for a 13-week period. Infusing SW 1: 250 (v:v) into half-strength MS medium significantly increased the germination rate index (GRI) and the development rate index (DRI) of the A. africana seeds. All the SW treatments significantly increased the number of large protocorm bodies at the final stage of development. Infusing KAR(1) into the growing medium had no significant effect on germination or development of the seeds. The TMB treatment, however, significantly reduced the GRI and DRI of A. africana seeds.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Biology

ISSN

1435-8603

e-ISSN

—

Svazek periodika

18

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

289-294

Kód UT WoS článku

000369977800013

EID výsledku v databázi Scopus

2-s2.0-84957839471