Protective hybrid coating containing silver, copper and zinc cations effective against human immunodeficiency virus and other enveloped viruses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00459232" target="_blank" >RIV/61388963:_____/16:00459232 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24510/16:00000665 RIV/46747885:24530/16:00000665 RIV/46747885:24620/16:00000665

Výsledek na webu

<a href="http://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0675-x" target="_blank" >http://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0675-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12866-016-0675-x" target="_blank" >10.1186/s12866-016-0675-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Protective hybrid coating containing silver, copper and zinc cations effective against human immunodeficiency virus and other enveloped viruses

Popis výsledku v původním jazyce

Healthcare-acquired infections by pathogenic microorganisms including viruses represent significant health concern worldwide. Next to direct transmission from person-to-person also indirect transmission from contaminated surfaces is well documented and important route of infections. Here, we tested antiviral properties of hybrid coating containing silver, copper and zinc cations that was previously shown to be effective against pathogenic bacteria including methicillin-resistant Staphylococcus aureus. Hybrid coatings containing silver, copper and zinc cations were prepared through radical polymerization via sol-gel method and applied on glass slides or into the wells of polymethylmethacrylate plates. A 10 mu l droplet of several viruses such as human immunodeficiency virus type 1 (HIV-1), influenza, dengue virus, herpes simplex virus, and coxsackievirus was added to coated and uncoated slides or plates, incubated usually from 5 to 240 min and followed by titer determination of recovered virus. Scanning electron microscopy analysis showed better adhesion of coatings on glass surfaces, which resulted in 99.5-100% HIV-1 titer reduction (3.1 +/- 0.8 log(10)TCID(50), n=3) already after 20 min of exposure to coatings, than on coated polymethylmethacrylate plates with 75-100% (1.7 +/- 1.1 log(10)TCID(50), n=3) and 98-100% (2.3 +/- 0.5 log(10)TCID(50), n=3) HIV-1 titer reduction after 20 and 120 min of exposure, respectively. Slower virucidal kinetics was observed with other enveloped viruses, where 240 min exposure to coated slides lead to 97% (dengue), 100% (herpes simplex) and 77% (influenza) reduction in virus titers. Interestingly, only marginal reduction in viral titer after 240 min of exposure was noticed for non-enveloped coxsackie B3 virus. Our hybrid coatings showed virucidal activity against HIV and other enveloped viruses thus providing further findings towards development of broad-spectrum antimicrobial coating suitable for surfaces in healthcare settings.

Název v anglickém jazyce

Protective hybrid coating containing silver, copper and zinc cations effective against human immunodeficiency virus and other enveloped viruses

Popis výsledku anglicky

Healthcare-acquired infections by pathogenic microorganisms including viruses represent significant health concern worldwide. Next to direct transmission from person-to-person also indirect transmission from contaminated surfaces is well documented and important route of infections. Here, we tested antiviral properties of hybrid coating containing silver, copper and zinc cations that was previously shown to be effective against pathogenic bacteria including methicillin-resistant Staphylococcus aureus. Hybrid coatings containing silver, copper and zinc cations were prepared through radical polymerization via sol-gel method and applied on glass slides or into the wells of polymethylmethacrylate plates. A 10 mu l droplet of several viruses such as human immunodeficiency virus type 1 (HIV-1), influenza, dengue virus, herpes simplex virus, and coxsackievirus was added to coated and uncoated slides or plates, incubated usually from 5 to 240 min and followed by titer determination of recovered virus. Scanning electron microscopy analysis showed better adhesion of coatings on glass surfaces, which resulted in 99.5-100% HIV-1 titer reduction (3.1 +/- 0.8 log(10)TCID(50), n=3) already after 20 min of exposure to coatings, than on coated polymethylmethacrylate plates with 75-100% (1.7 +/- 1.1 log(10)TCID(50), n=3) and 98-100% (2.3 +/- 0.5 log(10)TCID(50), n=3) HIV-1 titer reduction after 20 and 120 min of exposure, respectively. Slower virucidal kinetics was observed with other enveloped viruses, where 240 min exposure to coated slides lead to 97% (dengue), 100% (herpes simplex) and 77% (influenza) reduction in virus titers. Interestingly, only marginal reduction in viral titer after 240 min of exposure was noticed for non-enveloped coxsackie B3 virus. Our hybrid coatings showed virucidal activity against HIV and other enveloped viruses thus providing further findings towards development of broad-spectrum antimicrobial coating suitable for surfaces in healthcare settings.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Microbiology

ISSN

1471-2180

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

Apr 1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000373187300001

EID výsledku v databázi Scopus

2-s2.0-85008477487