Co-option of the sphingolipid metabolism for the production of nitroalkene defensive chemicals in termite soldiers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00475221" target="_blank" >RIV/61388963:_____/17:00475221 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/17:00475221

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ibmb.2017.01.008" target="_blank" >http://dx.doi.org/10.1016/j.ibmb.2017.01.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ibmb.2017.01.008" target="_blank" >10.1016/j.ibmb.2017.01.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Co-option of the sphingolipid metabolism for the production of nitroalkene defensive chemicals in termite soldiers

Popis výsledku v původním jazyce

The aliphatic nitroalkene (E)-1-nitropentadec-1-ene (NPD), reported in early seventies in soldiers of the termite genus Prorhinotermes, was the first documented nitro compound produced by insects. Yet, its biosynthetic origin has long remained unknown. Our observations provide a strong support to a biosynthetic scenario of NPD formation, representing an analogy of the sphingolipid pathway starting with the condensation of tetradecanoic acid with L-serine and leading to the formation of a C16 sphinganine. The proposed biosynthetic sequence has been constructed from age-related quantitative dynamics of individual intermediates and confirmed by the detection of labeled products downstream of the administered intermediates. Comparative RNA-Seq analyses followed by qRT-PCR validation identified orthologs of serine palmitoyltransferase and 3-ketodihydrosphingosine reductase genes as highly expressed in the NPD productionnsite.

Název v anglickém jazyce

Co-option of the sphingolipid metabolism for the production of nitroalkene defensive chemicals in termite soldiers

Popis výsledku anglicky

The aliphatic nitroalkene (E)-1-nitropentadec-1-ene (NPD), reported in early seventies in soldiers of the termite genus Prorhinotermes, was the first documented nitro compound produced by insects. Yet, its biosynthetic origin has long remained unknown. Our observations provide a strong support to a biosynthetic scenario of NPD formation, representing an analogy of the sphingolipid pathway starting with the condensation of tetradecanoic acid with L-serine and leading to the formation of a C16 sphinganine. The proposed biosynthetic sequence has been constructed from age-related quantitative dynamics of individual intermediates and confirmed by the detection of labeled products downstream of the administered intermediates. Comparative RNA-Seq analyses followed by qRT-PCR validation identified orthologs of serine palmitoyltransferase and 3-ketodihydrosphingosine reductase genes as highly expressed in the NPD productionnsite.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GP13-25137P" target="_blank" >GP13-25137P: Biosyntéza (E)-1-nitropentadec-1-enu u vojáků termitů rodu Prorhinotermes</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Insect Biochemistry and Molecular Biology

ISSN

0965-1748

e-ISSN

—

Svazek periodika

82

Číslo periodika v rámci svazku

Mar

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

52-61

Kód UT WoS článku

000397361500006

EID výsledku v databázi Scopus

2-s2.0-85011932304