Determination of selected bisphenols, parabens and estrogens in human plasma using LC-MS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00477766" target="_blank" >RIV/61388963:_____/17:00477766 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/17:00477766 RIV/61383082:_____/17:00000376 RIV/00216208:11110/17:10368056 RIV/00023761:_____/17:N0000017

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.talanta.2017.05.070" target="_blank" >http://dx.doi.org/10.1016/j.talanta.2017.05.070</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2017.05.070" target="_blank" >10.1016/j.talanta.2017.05.070</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of selected bisphenols, parabens and estrogens in human plasma using LC-MS/MS

Popis výsledku v původním jazyce

In this study, a novel liquid chromatography tandem mass spectrometry method for the simultaneous determination of bisphenols (BPA, BPS, BPF, BPAF), parabens (methyl-, ethyl-, propyl-, butyl-, benzyl-paraben) and estrogens (estrone, estradiol, estriol) in human plasma is presented. Since all analytes possess the phenolic group, dansyl chloride derivatization was applied in order to gain high sensitivity. The method was validated according to FDA guidelines, and all validation requirements were satisfactory. The lower limits of quantifications were 41.6, 54.9, 43.5 and 150.8 pg/mL for BPA, BPS, BPF and BPAF, 172, 149, 171, 134 and 202 pg/mL for methyl-, ethyl-, propyl-, butyl- and benzyl-paraben, 10.5, 6.7 and 9.4 pg/mL for estrone, estradiol and estriol, respectively. This is the first method allowing the determination of plasma bisphenols, parabens and estrogens in one run, and also the first determination of BPF levels in human plasma. The method was used to examine the plasma levels of healthy normospermic men, where three times higher plasma levels of BPF than BPA were found.

Název v anglickém jazyce

Determination of selected bisphenols, parabens and estrogens in human plasma using LC-MS/MS

Popis výsledku anglicky

In this study, a novel liquid chromatography tandem mass spectrometry method for the simultaneous determination of bisphenols (BPA, BPS, BPF, BPAF), parabens (methyl-, ethyl-, propyl-, butyl-, benzyl-paraben) and estrogens (estrone, estradiol, estriol) in human plasma is presented. Since all analytes possess the phenolic group, dansyl chloride derivatization was applied in order to gain high sensitivity. The method was validated according to FDA guidelines, and all validation requirements were satisfactory. The lower limits of quantifications were 41.6, 54.9, 43.5 and 150.8 pg/mL for BPA, BPS, BPF and BPAF, 172, 149, 171, 134 and 202 pg/mL for methyl-, ethyl-, propyl-, butyl- and benzyl-paraben, 10.5, 6.7 and 9.4 pg/mL for estrone, estradiol and estriol, respectively. This is the first method allowing the determination of plasma bisphenols, parabens and estrogens in one run, and also the first determination of BPF levels in human plasma. The method was used to examine the plasma levels of healthy normospermic men, where three times higher plasma levels of BPF than BPA were found.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

—

Svazek periodika

174

Číslo periodika v rámci svazku

NOV 1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

21-28

Kód UT WoS článku

000407524900004

EID výsledku v databázi Scopus

2-s2.0-85019970353