Cholesteryl esters of omega-(O-acyl)-hydroxy fatty acids in vernix caseosa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00478141" target="_blank" >RIV/61388963:_____/17:00478141 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/17:10364232 RIV/00216208:11310/17:10364232 RIV/00064165:_____/17:10364232

Výsledek na webu

<a href="http://dx.doi.org/10.1194/jlr.M075333" target="_blank" >http://dx.doi.org/10.1194/jlr.M075333</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1194/jlr.M075333" target="_blank" >10.1194/jlr.M075333</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cholesteryl esters of omega-(O-acyl)-hydroxy fatty acids in vernix caseosa

Popis výsledku v původním jazyce

Cholesteryl esters of omega-(O-acyl)-hydroxy FAs (Chl. OAHFAs) were identified for the first time in vernix caseosa and characterized using chromatography and MS. Chl-omega OAHFAs were isolated using adsorption chromatography on silica gel and magnesium hydroxide. Their general structure was established using high-resolution and tandem MS of intact lipids, and products of their transesterification and derivatizations. Individual molecular species were characterized using nonaqueous reversed-phase HPLC coupled to atmospheric pressure chemical ionization. The analytes were detected as protonated molecules, and their structures were elucidated in the negative ion mode using controlled thermal decomposition and data-dependent fragmentation. About three hundred molecular species of Chl-omega OAHFAs were identified in this way. The most abundant Chl. OAHFAs contained 32:1 omega-hydroxy FA (omega-HFA) and 14:0, 15:0, 16:0, 16:1, and 18:1 FAs. The double bond in the 32:1 omega-HFA was in the n-7 and n-9 positions. Chl-omega OAHFAs are estimated to account for approximately 1-2% of vernix caseosa lipids.

Název v anglickém jazyce

Cholesteryl esters of omega-(O-acyl)-hydroxy fatty acids in vernix caseosa

Popis výsledku anglicky

Cholesteryl esters of omega-(O-acyl)-hydroxy FAs (Chl. OAHFAs) were identified for the first time in vernix caseosa and characterized using chromatography and MS. Chl-omega OAHFAs were isolated using adsorption chromatography on silica gel and magnesium hydroxide. Their general structure was established using high-resolution and tandem MS of intact lipids, and products of their transesterification and derivatizations. Individual molecular species were characterized using nonaqueous reversed-phase HPLC coupled to atmospheric pressure chemical ionization. The analytes were detected as protonated molecules, and their structures were elucidated in the negative ion mode using controlled thermal decomposition and data-dependent fragmentation. About three hundred molecular species of Chl-omega OAHFAs were identified in this way. The most abundant Chl. OAHFAs contained 32:1 omega-hydroxy FA (omega-HFA) and 14:0, 15:0, 16:0, 16:1, and 18:1 FAs. The double bond in the 32:1 omega-HFA was in the n-7 and n-9 positions. Chl-omega OAHFAs are estimated to account for approximately 1-2% of vernix caseosa lipids.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GAP206%2F12%2F0750" target="_blank" >GAP206/12/0750: Lipidom novorozeneckého mázku: komplexní analýza a biologická variabilita</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Lipid Research

ISSN

0022-2275

e-ISSN

—

Svazek periodika

58

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

1579-1590

Kód UT WoS článku

000406686600009

EID výsledku v databázi Scopus

2-s2.0-85026740185