LC-MS/MS analysis of lipidized analogs of prolactin-releasing peptide utilizing a monolithic column and simple sample preparation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00480170" target="_blank" >RIV/61388963:_____/17:00480170 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/17:43913173

Výsledek na webu

<a href="http://dx.doi.org/10.4155/bio-2017-0125" target="_blank" >http://dx.doi.org/10.4155/bio-2017-0125</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4155/bio-2017-0125" target="_blank" >10.4155/bio-2017-0125</a>

Jazyk výsledku

angličtina

Název v původním jazyce

LC-MS/MS analysis of lipidized analogs of prolactin-releasing peptide utilizing a monolithic column and simple sample preparation

Popis výsledku v původním jazyce

Novel compounds for obesity treatment are currently being studied employing lipidized analogs of anorexigenic neuropeptides. Various analogs of prolactin-releasing peptide have demonstrated their ability to decrease food intake. Adequate analytical tools are required to support corresponding research. An analytical method was developed that includes simple dilution of plasma samples prior to liquid chromatography-mass spectrometry and employs a monolithic column for the determination of lipidized analogs of prolactin-releasing peptide in complex biological samples. A multiple reaction monitoring approach was applied that included matrix calibration and an internal standard and produced a linear calibration range 20-200 ng ml(-1) in rat and macaque plasma samples. A straightforward, simple and reliable analytical method was developed satisfying major validation criteria.

Název v anglickém jazyce

LC-MS/MS analysis of lipidized analogs of prolactin-releasing peptide utilizing a monolithic column and simple sample preparation

Popis výsledku anglicky

Novel compounds for obesity treatment are currently being studied employing lipidized analogs of anorexigenic neuropeptides. Various analogs of prolactin-releasing peptide have demonstrated their ability to decrease food intake. Adequate analytical tools are required to support corresponding research. An analytical method was developed that includes simple dilution of plasma samples prior to liquid chromatography-mass spectrometry and employs a monolithic column for the determination of lipidized analogs of prolactin-releasing peptide in complex biological samples. A multiple reaction monitoring approach was applied that included matrix calibration and an internal standard and produced a linear calibration range 20-200 ng ml(-1) in rat and macaque plasma samples. A straightforward, simple and reliable analytical method was developed satisfying major validation criteria.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bioanalysis

ISSN

1757-6180

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1319-1328

Kód UT WoS článku

000411492600006

EID výsledku v databázi Scopus

2-s2.0-85029793179