Application of capillary electromigration methods for physicochemical measurements

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00492951" target="_blank" >RIV/61388963:_____/18:00492951 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/B978-0-12-809375-7.00024-1" target="_blank" >http://dx.doi.org/10.1016/B978-0-12-809375-7.00024-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/B978-0-12-809375-7.00024-1" target="_blank" >10.1016/B978-0-12-809375-7.00024-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of capillary electromigration methods for physicochemical measurements

Popis výsledku v původním jazyce

In this chapter, high-performance capillary electromigration (HPCE or CE) methods are presented as powerful tools for the determination of important physicochemical parameters of (bio)molecules. Using the different HPCE methods (zone electrophoresis in free solutions or in sieving/gel media, isotachophoresis, isoelectric focusing, affinity electrophoresis, and electrokinetic chromatography), the following physicochemical characteristics of a wide spectrum of compounds can be determined: effective, ionic and limiting mobilities, effective charges, isoelectric points, Stokes radii, relative molecular masses, partition constants, diffusion coefficients, and acidity (ionization) constants (pKa). In addition, the binding (stability, association, formation, or dissociation) constants of (bio)molecular complexes and rate constants of chemical and enzymatic reactions and noncovalent molecular interactions can be estimated. Advantages of HPCE methods for these physicochemical measurements include high separation efficiency, short analysis time, and especially the ultra-small mass (picogram range) and volume (nanoliter level) of injected solute samples and low consumption of chemicals and solvents. Moreover, the characterization of not quite pure solutes and solutes sparingly soluble in water is possible.

Název v anglickém jazyce

Application of capillary electromigration methods for physicochemical measurements

Popis výsledku anglicky

In this chapter, high-performance capillary electromigration (HPCE or CE) methods are presented as powerful tools for the determination of important physicochemical parameters of (bio)molecules. Using the different HPCE methods (zone electrophoresis in free solutions or in sieving/gel media, isotachophoresis, isoelectric focusing, affinity electrophoresis, and electrokinetic chromatography), the following physicochemical characteristics of a wide spectrum of compounds can be determined: effective, ionic and limiting mobilities, effective charges, isoelectric points, Stokes radii, relative molecular masses, partition constants, diffusion coefficients, and acidity (ionization) constants (pKa). In addition, the binding (stability, association, formation, or dissociation) constants of (bio)molecular complexes and rate constants of chemical and enzymatic reactions and noncovalent molecular interactions can be estimated. Advantages of HPCE methods for these physicochemical measurements include high separation efficiency, short analysis time, and especially the ultra-small mass (picogram range) and volume (nanoliter level) of injected solute samples and low consumption of chemicals and solvents. Moreover, the characterization of not quite pure solutes and solutes sparingly soluble in water is possible.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Capillary Electromigration Separation Methods

ISBN

978-0-12-809375-7

Počet stran výsledku

45

Strana od-do

547-591

Počet stran knihy

626

Název nakladatele

Elsevier

Místo vydání

Amsterdam

Kód UT WoS kapitoly

000462135300022