Effects of sulfation and the environment on the structure of chondroitin sulfate studied via Raman optical activity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00504471" target="_blank" >RIV/61388963:_____/19:00504471 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11320/19:10406255

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlelanding/2019/CP/C9CP00472F#!divAbstract" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2019/CP/C9CP00472F#!divAbstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c9cp00472f" target="_blank" >10.1039/c9cp00472f</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of sulfation and the environment on the structure of chondroitin sulfate studied via Raman optical activity

Popis výsledku v původním jazyce

Glycosaminoglycans are linear carbohydrate polymers with essential roles in many biological processes. Chondroitin sulfate (CS) is one of them, omnipresent in living organisms as an important structural component of cartilage. It provides much of its resistance to compression. Despite its biological importance, little is still known about the relation of the CS structure to chemical composition and interaction with the environment. We therefore measured Raman and Raman optical activity (ROA) spectra of five CS samples of different biological origin and variously sulfated CS building blocks (GlcA, GalNAc, and basic disaccharide units) in a wide frequency range between 200 cm(-1) and 1800 cm(-1) and analyzed them with respect to specific structure marker bands. We show that ROA spectroscopy is sensitive to the conformational stability and rigidity of pyranose rings of saccharides, the orientation of sugar hydroxyl groups and the secondary structure of the CS's backbone. The CS secondary structure has been found to be quite stable, with a minor variation as a reaction to physicochemical parameters (concentration, pH, temperature, and the presence of cations). Larger changes were observed under chemical changes (sulfation) of the CS chain. ROA spectroscopy thus exhibited useful potential to study the structure of similar biopolymers.

Název v anglickém jazyce

Effects of sulfation and the environment on the structure of chondroitin sulfate studied via Raman optical activity

Popis výsledku anglicky

Glycosaminoglycans are linear carbohydrate polymers with essential roles in many biological processes. Chondroitin sulfate (CS) is one of them, omnipresent in living organisms as an important structural component of cartilage. It provides much of its resistance to compression. Despite its biological importance, little is still known about the relation of the CS structure to chemical composition and interaction with the environment. We therefore measured Raman and Raman optical activity (ROA) spectra of five CS samples of different biological origin and variously sulfated CS building blocks (GlcA, GalNAc, and basic disaccharide units) in a wide frequency range between 200 cm(-1) and 1800 cm(-1) and analyzed them with respect to specific structure marker bands. We show that ROA spectroscopy is sensitive to the conformational stability and rigidity of pyranose rings of saccharides, the orientation of sugar hydroxyl groups and the secondary structure of the CS's backbone. The CS secondary structure has been found to be quite stable, with a minor variation as a reaction to physicochemical parameters (concentration, pH, temperature, and the presence of cations). Larger changes were observed under chemical changes (sulfation) of the CS chain. ROA spectroscopy thus exhibited useful potential to study the structure of similar biopolymers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physical Chemistry Chemical Physics

ISSN

1463-9076

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

7367-7377

Kód UT WoS článku

000464580600011

EID výsledku v databázi Scopus

2-s2.0-85063994384