Antimicrobial peptides prevent bacterial biofilm formation on the surface of polymethylmethacrylate bone cement

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00505762" target="_blank" >RIV/61388963:_____/19:00505762 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10394756 RIV/00064203:_____/19:10394756

Výsledek na webu

<a href="https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001000" target="_blank" >https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001000</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/jmm.0.001000" target="_blank" >10.1099/jmm.0.001000</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antimicrobial peptides prevent bacterial biofilm formation on the surface of polymethylmethacrylate bone cement

Popis výsledku v původním jazyce

Purpose: Antibiotic-loaded polymethylmethacrylate-based bone cement has been implemented in orthopaedics to cope with implant-related infections associated with the formation of bacterial biofilms. In the context of emerging bacterial resistance to current antibiotics, we examined the efficacy of short antimicrobial peptide-loaded bone cement in inhibiting bacterial adhesion and consequent biofilm formation on its surface. Methodology: The ability of alpha-helical antimicrobial peptides composed of 12 amino acid residues to prevent bacterial biofilm [methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli] formation on the surface of model implants made from polymethylmethacrylate-based bone cement was evaluated by colony-forming unit (c.f) counting of bacteria released by sonication from the biofilms formed on their surfaces. The biofilms on model implant surfaces were also visualized by light microscopy after staining with tetrazolium dye (MTT) and by scanning electron microscopy. Results: When incorporated in the implants, these peptides caused a mean reduction in the number of bacterial cells attached to implants' surfaces (by five orders of magnitude), and 88 % of these implants showed no bacterial adhesion after being exposed to growth media containing various bacteria. Conclusion: The results showed that the antibiofilm activity of these peptides was comparable to that of the antibiotics, but the peptides exhibited broader specificity than the antibiotics. Given the rapid development of antibiotic resistance, antimicrobial peptides show promise as a substitute for antibiotics for loading into bone cements.

Název v anglickém jazyce

Antimicrobial peptides prevent bacterial biofilm formation on the surface of polymethylmethacrylate bone cement

Popis výsledku anglicky

Purpose: Antibiotic-loaded polymethylmethacrylate-based bone cement has been implemented in orthopaedics to cope with implant-related infections associated with the formation of bacterial biofilms. In the context of emerging bacterial resistance to current antibiotics, we examined the efficacy of short antimicrobial peptide-loaded bone cement in inhibiting bacterial adhesion and consequent biofilm formation on its surface. Methodology: The ability of alpha-helical antimicrobial peptides composed of 12 amino acid residues to prevent bacterial biofilm [methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli] formation on the surface of model implants made from polymethylmethacrylate-based bone cement was evaluated by colony-forming unit (c.f) counting of bacteria released by sonication from the biofilms formed on their surfaces. The biofilms on model implant surfaces were also visualized by light microscopy after staining with tetrazolium dye (MTT) and by scanning electron microscopy. Results: When incorporated in the implants, these peptides caused a mean reduction in the number of bacterial cells attached to implants' surfaces (by five orders of magnitude), and 88 % of these implants showed no bacterial adhesion after being exposed to growth media containing various bacteria. Conclusion: The results showed that the antibiofilm activity of these peptides was comparable to that of the antibiotics, but the peptides exhibited broader specificity than the antibiotics. Given the rapid development of antibiotic resistance, antimicrobial peptides show promise as a substitute for antibiotics for loading into bone cements.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV16-27726A" target="_blank" >NV16-27726A: Nové antimikrobiální peptidy pro lokální léčbu osteomyelitidy a prevenci infekcí implantátů v ortopedii</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Medical Microbiology

ISSN

0022-2615

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

961-972

Kód UT WoS článku

000470083100019

EID výsledku v databázi Scopus

2-s2.0-85067089662