Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00509114" target="_blank" >RIV/61388963:_____/19:00509114 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41330/19:81523

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900043" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/elps.201900043</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201900043" target="_blank" >10.1002/elps.201900043</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol

Popis výsledku v původním jazyce

Affinity capillary electrophoresis (ACE) and pressure-assisted ACE were employed to study the noncovalent molecular interactions of antamanide (AA), cyclic decapeptide from the deadly poisonous fungus Amanita phalloides, with univalent (Li+, Na+, K+, and NH4+) and divalent (Mg2+ and Ca2+) cations in methanol. The strength of these interactions was quantified by the apparent stability constants of the appropriate AA-cation complexes. The stability constants were calculated using the nonlinear regression analysis of the dependence of the effective electrophoretic mobility of AA on the concentration of the above ions in the BGE (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pH(MeOH) 7.8, containing 0-50 mM concentrations of the above ions added in the form of chlorides). Prior to stability constant calculation, the AA effective mobilities measured at actual temperature inside the capillary and at variable ionic strength of the BGEs were corrected to the values corresponding to the reference temperature of 25 degrees C and to the constant ionic strength of 10 mM. From the above ions, sodium cation interacted with AA moderately strong with the stability constant 362 +/- 16 L/mol. K+, Mg2+, and Ca2+ cations formed with AA weak complexes with stability constants in the range 37-31 L/mol decreasing in the order K+ > Ca2+ > Mg2+. No interactions were observed between AA and small Li+ and large NH4+ cations.

Název v anglickém jazyce

Affinity capillary electrophoresis employed for determination of stability constants of antamanide complexes with univalent and divalent cations in methanol

Popis výsledku anglicky

Affinity capillary electrophoresis (ACE) and pressure-assisted ACE were employed to study the noncovalent molecular interactions of antamanide (AA), cyclic decapeptide from the deadly poisonous fungus Amanita phalloides, with univalent (Li+, Na+, K+, and NH4+) and divalent (Mg2+ and Ca2+) cations in methanol. The strength of these interactions was quantified by the apparent stability constants of the appropriate AA-cation complexes. The stability constants were calculated using the nonlinear regression analysis of the dependence of the effective electrophoretic mobility of AA on the concentration of the above ions in the BGE (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pH(MeOH) 7.8, containing 0-50 mM concentrations of the above ions added in the form of chlorides). Prior to stability constant calculation, the AA effective mobilities measured at actual temperature inside the capillary and at variable ionic strength of the BGEs were corrected to the values corresponding to the reference temperature of 25 degrees C and to the constant ionic strength of 10 mM. From the above ions, sodium cation interacted with AA moderately strong with the stability constant 362 +/- 16 L/mol. K+, Mg2+, and Ca2+ cations formed with AA weak complexes with stability constants in the range 37-31 L/mol decreasing in the order K+ > Ca2+ > Mg2+. No interactions were observed between AA and small Li+ and large NH4+ cations.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

40

Číslo periodika v rámci svazku

18/19

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

2321-2328

Kód UT WoS článku

000483858400010

EID výsledku v databázi Scopus

2-s2.0-85065993665