Increasing the throughput of crystallization condition screens: Challenges and pitfalls of acoustic dispensing systems

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00509613" target="_blank" >RIV/61388963:_____/19:00509613 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2215016119302547?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2215016119302547?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mex.2019.09.030" target="_blank" >10.1016/j.mex.2019.09.030</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Increasing the throughput of crystallization condition screens: Challenges and pitfalls of acoustic dispensing systems

Popis výsledku v původním jazyce

Advances in contactless acoustic liquid transfer technologies have unlocked opportunities to substantially increase the throughput of crystallization screens and decrease the consumption of reagents and consumables. Acoustic energy transfer enables crystallization experiments to be set up precisely and rapidly on a nanoliter scale. Nonetheless, adapting acoustic transfer methods to a diverse range of crystallization conditions and their physicochemical idiosyncrasies remains a major bottleneck for true universality of this technique. Even though the reagent limitations still remain an issue, we present a straightforward protocol for setting up crystallization experiments by acoustic transfer using a Labcyte Echo 550 instrument, with a focus on the technical limitations of this method, including reagent compatibilities, spatial resolution and downscaling limits.

Název v anglickém jazyce

Increasing the throughput of crystallization condition screens: Challenges and pitfalls of acoustic dispensing systems

Popis výsledku anglicky

Advances in contactless acoustic liquid transfer technologies have unlocked opportunities to substantially increase the throughput of crystallization screens and decrease the consumption of reagents and consumables. Acoustic energy transfer enables crystallization experiments to be set up precisely and rapidly on a nanoliter scale. Nonetheless, adapting acoustic transfer methods to a diverse range of crystallization conditions and their physicochemical idiosyncrasies remains a major bottleneck for true universality of this technique. Even though the reagent limitations still remain an issue, we present a straightforward protocol for setting up crystallization experiments by acoustic transfer using a Labcyte Echo 550 instrument, with a focus on the technical limitations of this method, including reagent compatibilities, spatial resolution and downscaling limits.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MethodsX

ISSN

2215-0161

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

Sep 25

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

2230-2236

Kód UT WoS článku

000493729600242

EID výsledku v databázi Scopus

2-s2.0-85072793405