Measuring venous-arterial differences of valine, isoleucine, leucine, alanine and glutamine in skeletal muscles using counter-current electrophoresis with contactless conductivity detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00519392" target="_blank" >RIV/61388963:_____/20:00519392 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/20:43919519 RIV/00064173:_____/20:N0000045

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1572665719310409?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1572665719310409?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2019.113772" target="_blank" >10.1016/j.jelechem.2019.113772</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Measuring venous-arterial differences of valine, isoleucine, leucine, alanine and glutamine in skeletal muscles using counter-current electrophoresis with contactless conductivity detection

Popis výsledku v původním jazyce

Plasmatic levels of valine, isoleucine, leucine, alanine and glutamine were determined by capillary electrophoresis with contactless conductivity detection. A new kind of covalently coated capillary based on polyacrylamide and (3-acrylamidopropyl) trimethylammonium chloride was employed. The amino acids were separated in the counter-current regime, in which the analytes migrate under counter-current electro-osmotic flow with a value of 5.53 · 10−9 m2 V−1 s−1 in the background electrolyte, 3.2 mol/L AcOH +10% v/v MeOH. The baseline separation of all five amino acids from other plasmatic compounds was achieved in <5 min, the limits of detections are 0.13–0.14 μmol/L for the treated sample and 0.50–0.58 μmol/L for the untreated plasma. The method was used to measure venous-arterial differences of amino acids in hyperinsulinemic patients with cachexia.

Název v anglickém jazyce

Measuring venous-arterial differences of valine, isoleucine, leucine, alanine and glutamine in skeletal muscles using counter-current electrophoresis with contactless conductivity detection

Popis výsledku anglicky

Plasmatic levels of valine, isoleucine, leucine, alanine and glutamine were determined by capillary electrophoresis with contactless conductivity detection. A new kind of covalently coated capillary based on polyacrylamide and (3-acrylamidopropyl) trimethylammonium chloride was employed. The amino acids were separated in the counter-current regime, in which the analytes migrate under counter-current electro-osmotic flow with a value of 5.53 · 10−9 m2 V−1 s−1 in the background electrolyte, 3.2 mol/L AcOH +10% v/v MeOH. The baseline separation of all five amino acids from other plasmatic compounds was achieved in <5 min, the limits of detections are 0.13–0.14 μmol/L for the treated sample and 0.50–0.58 μmol/L for the untreated plasma. The method was used to measure venous-arterial differences of amino acids in hyperinsulinemic patients with cachexia.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

—

Svazek periodika

857

Číslo periodika v rámci svazku

Jan 15

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

6

Strana od-do

113772

Kód UT WoS článku

000518494300030

EID výsledku v databázi Scopus

2-s2.0-85076996162