The bio-chemically selective interaction of hydrogenated and oxidized ultra-small nanodiamonds with proteins and cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00523791" target="_blank" >RIV/61388963:_____/20:00523791 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378271:_____/20:00523791 RIV/68407700:21230/20:00342539 RIV/00216208:11110/20:10410730 RIV/00216208:11140/20:10410730 a 2 dalších

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0008622320302074" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0008622320302074</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carbon.2020.02.061" target="_blank" >10.1016/j.carbon.2020.02.061</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The bio-chemically selective interaction of hydrogenated and oxidized ultra-small nanodiamonds with proteins and cells

Popis výsledku v původním jazyce

Ultra-small nanoparticles of a size smaller than or comparable to cell membrane pores (1–5 nm) offer significant potential in the field of biomedicine. This study presents a systematic in vitro investigation of fundamental bio-chemical interactions of such ultra-small hydrogenated and oxidized detonation nanodiamonds (DNDs) with biomolecules and human cells. We apply mass spectrometry methods (LC-MS/MS) for the qualitative and quantitative analysis of the protein corona as a function of the surface chemistry and size of DNDs. We observe that protein interactions with DNDs are more related to their surface chemistry (H/O-termination) rather than size. Bioinformatics characterization of the identified proteins points to the strong influence of electrostatic interaction between proteins and DNDs depending on their termination. Such specific interaction leads to formation of different protein corona on 2 nm DNDs, which influences also interaction with cells including different level of cytotoxicity.

Název v anglickém jazyce

The bio-chemically selective interaction of hydrogenated and oxidized ultra-small nanodiamonds with proteins and cells

Popis výsledku anglicky

Ultra-small nanoparticles of a size smaller than or comparable to cell membrane pores (1–5 nm) offer significant potential in the field of biomedicine. This study presents a systematic in vitro investigation of fundamental bio-chemical interactions of such ultra-small hydrogenated and oxidized detonation nanodiamonds (DNDs) with biomolecules and human cells. We apply mass spectrometry methods (LC-MS/MS) for the qualitative and quantitative analysis of the protein corona as a function of the surface chemistry and size of DNDs. We observe that protein interactions with DNDs are more related to their surface chemistry (H/O-termination) rather than size. Bioinformatics characterization of the identified proteins points to the strong influence of electrostatic interaction between proteins and DNDs depending on their termination. Such specific interaction leads to formation of different protein corona on 2 nm DNDs, which influences also interaction with cells including different level of cytotoxicity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Carbon

ISSN

0008-6223

e-ISSN

—

Svazek periodika

162

Číslo periodika v rámci svazku

Jun

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

650-661

Kód UT WoS článku

000526113000067

EID výsledku v databázi Scopus

2-s2.0-85082057943