Conventional, Apodized, and Relief Phase-Contrast Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00531526" target="_blank" >RIV/61388963:_____/20:00531526 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/20:00552439

Výsledek na webu

<a href="https://doi.org/10.1007/978-1-0716-0428-1_10" target="_blank" >https://doi.org/10.1007/978-1-0716-0428-1_10</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-0716-0428-1_10" target="_blank" >10.1007/978-1-0716-0428-1_10</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Conventional, Apodized, and Relief Phase-Contrast Microscopy

Popis výsledku v původním jazyce

Non-absorbing colorless specimens (phase objects) can be visualized either by chemical staining (histology) or by the so-called optical contrasting (staining). The latter is achieved by converting optical phase shifts within the specimen, invisible to human eye, to intensity differences in the microscopic image. Out of several available modes of microscopic phase visualization, conventional, apodized, and relief phase contrast are described in detail. A comparison with relief contrast alone, that is, the off-axis (schlieren) illumination mode is presented as well. Images of various phase specimens of biological origin are shown, demonstrating the strong and weak points of each mode. Physiological aspects of image comprehension, facilitated by shading and other visual cues to depth structure are briefly discussed. A phase-contrast microscope equipped with an objective hosting no phase annulus is presented, the latter is located in a pupil projection (optically relayed) plane, in an external attachment unit. This setup enables phase-contrast and, for example, conventional epi-fluorescence and total internal reflection fluorescence (TIRF) images to be acquired with a single objective lens. Such modality is demonstrated in growth cones of neuroblastoma–glioma hybrid mouse–rat cells and touch receptor neurons of Caenorhabditis elegans. Examples of phase-contrast imaging in electron and X-ray (synchrotron radiation) microscopy are also presented.

Název v anglickém jazyce

Conventional, Apodized, and Relief Phase-Contrast Microscopy

Popis výsledku anglicky

Non-absorbing colorless specimens (phase objects) can be visualized either by chemical staining (histology) or by the so-called optical contrasting (staining). The latter is achieved by converting optical phase shifts within the specimen, invisible to human eye, to intensity differences in the microscopic image. Out of several available modes of microscopic phase visualization, conventional, apodized, and relief phase contrast are described in detail. A comparison with relief contrast alone, that is, the off-axis (schlieren) illumination mode is presented as well. Images of various phase specimens of biological origin are shown, demonstrating the strong and weak points of each mode. Physiological aspects of image comprehension, facilitated by shading and other visual cues to depth structure are briefly discussed. A phase-contrast microscope equipped with an objective hosting no phase annulus is presented, the latter is located in a pupil projection (optically relayed) plane, in an external attachment unit. This setup enables phase-contrast and, for example, conventional epi-fluorescence and total internal reflection fluorescence (TIRF) images to be acquired with a single objective lens. Such modality is demonstrated in growth cones of neuroblastoma–glioma hybrid mouse–rat cells and touch receptor neurons of Caenorhabditis elegans. Examples of phase-contrast imaging in electron and X-ray (synchrotron radiation) microscopy are also presented.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Neurohistology and Imaging Techniques

ISBN

978-1-0716-0426-7

Počet stran výsledku

49

Strana od-do

275-323

Počet stran knihy

472

Název nakladatele

Springer

Místo vydání

New York

Kód UT WoS kapitoly

—