Naloxone Is a Potential Binding Ligand and Activator of the Capsaicin Receptor TRPV1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00532415" target="_blank" >RIV/61388963:_____/20:00532415 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/20:10415787

Výsledek na webu

<a href="https://doi.org/10.1248/bpb.b19-00806" target="_blank" >https://doi.org/10.1248/bpb.b19-00806</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1248/bpb.b19-00806" target="_blank" >10.1248/bpb.b19-00806</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Naloxone Is a Potential Binding Ligand and Activator of the Capsaicin Receptor TRPV1

Popis výsledku v původním jazyce

The receptor channel transient receptor potential vanilloid 1 (TRPV1) functions as a sensor of noxious heat and various chemicals. There is increasing evidence for a crosstalk between TRPV1 and opioid receptors. Here we investigated the effect of the prototypical TRPV1 agonist capsaicin and selected opioid ligands on TRPV1 movement in the plasma membrane and intracellular calcium levels in HEK293 cells expressing TRPV1 tagged with cyan fluorescent protein (CFP). We observed that lateral mobility of TRPV1 increased after treatment of cells with capsaicin or naloxone (a nonselective opioid receptor antagonist) but not with DAMGO (a μ-opioid receptor agonist). Interestingly, both capsaicin and naloxone, unlike DAMGO, elicited intracellular calcium responses. The increased TRPV1 movement and calcium influx induced by capsaicin and naloxone were blocked by the TRPV1 antagonist capsazepine. The ability of naloxone to directly interact with TRPV1 was further corroborated by [3H]-naloxone binding. In conclusion, our data suggest that besides acting as an opioid receptor antagonist, naloxone may function as a potential TRPV1 agonist.

Název v anglickém jazyce

Naloxone Is a Potential Binding Ligand and Activator of the Capsaicin Receptor TRPV1

Popis výsledku anglicky

The receptor channel transient receptor potential vanilloid 1 (TRPV1) functions as a sensor of noxious heat and various chemicals. There is increasing evidence for a crosstalk between TRPV1 and opioid receptors. Here we investigated the effect of the prototypical TRPV1 agonist capsaicin and selected opioid ligands on TRPV1 movement in the plasma membrane and intracellular calcium levels in HEK293 cells expressing TRPV1 tagged with cyan fluorescent protein (CFP). We observed that lateral mobility of TRPV1 increased after treatment of cells with capsaicin or naloxone (a nonselective opioid receptor antagonist) but not with DAMGO (a μ-opioid receptor agonist). Interestingly, both capsaicin and naloxone, unlike DAMGO, elicited intracellular calcium responses. The increased TRPV1 movement and calcium influx induced by capsaicin and naloxone were blocked by the TRPV1 antagonist capsazepine. The ability of naloxone to directly interact with TRPV1 was further corroborated by [3H]-naloxone binding. In conclusion, our data suggest that besides acting as an opioid receptor antagonist, naloxone may function as a potential TRPV1 agonist.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biological & Pharmaceutical Bulletin

ISSN

0918-6158

e-ISSN

—

Svazek periodika

43

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

5

Strana od-do

908-912

Kód UT WoS článku

000567167900020

EID výsledku v databázi Scopus

2-s2.0-85084328535