Lipid Nanoparticles for Broad-Spectrum Nucleic Acid Delivery

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00545469" target="_blank" >RIV/61388963:_____/21:00545469 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/21:00545469 RIV/00216208:11110/21:10430494 RIV/00216208:11310/21:10430494

Výsledek na webu

<a href="https://doi.org/10.1002/adfm.202101391" target="_blank" >https://doi.org/10.1002/adfm.202101391</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/adfm.202101391" target="_blank" >10.1002/adfm.202101391</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Lipid Nanoparticles for Broad-Spectrum Nucleic Acid Delivery

Popis výsledku v původním jazyce

Lipid nanoparticles (LNPs) are the most advanced nonviral modality for nucleic acid (NA) delivery, and have recently gained enormous attention in the fields of RNA therapeutics and vaccine development. Here, ionizable adamantane-based lipidoids named XMaNs, which circumvent the usual need for laborious optimization of LNP components for highly diverse types of NAs, are described. The non-toxic XMaN6 lipidoid is highly versatile in entrapment and delivery of siRNA, mRNA, plasmid DNA, and a cyclic dinucleotide. XMaN6-based LNPs efficiently deliver: 1) siRNA into human primary hepatocytes and cell lines that are hard-to-transfect, 2) mRNA into mouse liver, 3) plasmid DNA, 4) 2′,3′-cGAMP into cells and activated the cGAS-STING pathway three orders of magnitude more efficiently than 2′,3′-cGAMP alone. To our knowledge, such universality in delivering different NA types has not been previously described and can accelerate translation of LNPs into the clinic.

Název v anglickém jazyce

Lipid Nanoparticles for Broad-Spectrum Nucleic Acid Delivery

Popis výsledku anglicky

Lipid nanoparticles (LNPs) are the most advanced nonviral modality for nucleic acid (NA) delivery, and have recently gained enormous attention in the fields of RNA therapeutics and vaccine development. Here, ionizable adamantane-based lipidoids named XMaNs, which circumvent the usual need for laborious optimization of LNP components for highly diverse types of NAs, are described. The non-toxic XMaN6 lipidoid is highly versatile in entrapment and delivery of siRNA, mRNA, plasmid DNA, and a cyclic dinucleotide. XMaN6-based LNPs efficiently deliver: 1) siRNA into human primary hepatocytes and cell lines that are hard-to-transfect, 2) mRNA into mouse liver, 3) plasmid DNA, 4) 2′,3′-cGAMP into cells and activated the cGAS-STING pathway three orders of magnitude more efficiently than 2′,3′-cGAMP alone. To our knowledge, such universality in delivering different NA types has not been previously described and can accelerate translation of LNPs into the clinic.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced Functional Materials

ISSN

1616-301X

e-ISSN

1616-3028

Svazek periodika

31

Číslo periodika v rámci svazku

47

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

2101391

Kód UT WoS článku

000688518800001

EID výsledku v databázi Scopus

2-s2.0-85113371724