Peptidomics and Capillary Electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00547345" target="_blank" >RIV/61388963:_____/21:00547345 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/978-3-030-77252-9_5" target="_blank" >https://doi.org/10.1007/978-3-030-77252-9_5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-030-77252-9_5" target="_blank" >10.1007/978-3-030-77252-9_5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Peptidomics and Capillary Electrophoresis

Popis výsledku v původním jazyce

Peptides play a crucial role in many vitally important functions of living organisms. The goal of peptidomics is the identification of the “peptidome,” the whole peptide content of a cell, organ, tissue, body fluid, or organism. In peptidomic or proteomic studies, capillary electrophoresis (CE) is an alternative technique for liquid chromatography. It is a highly efficient and fast separation method requiring extremely low amounts of sample. In peptidomic approaches, CE is commonly combined with mass spectrometric (MS) detection. Most often, CE is coupled with electrospray ionization MS and less frequently with matrix-assisted laser desorption/ionization MS. CE-MS has been employed in numerous studies dealing with determination of peptide biomarkers in different body fluids for various diseases, or in food peptidomic research for the analysis and identification of peptides with special biological activities. In addition to the above topics, sample preparation techniques commonly applied in peptidomics before CE separation and possibilities for peptide identification and quantification by CE-MS or CE-MS/MS methods are discussed in this chapter.

Název v anglickém jazyce

Peptidomics and Capillary Electrophoresis

Popis výsledku anglicky

Peptides play a crucial role in many vitally important functions of living organisms. The goal of peptidomics is the identification of the “peptidome,” the whole peptide content of a cell, organ, tissue, body fluid, or organism. In peptidomic or proteomic studies, capillary electrophoresis (CE) is an alternative technique for liquid chromatography. It is a highly efficient and fast separation method requiring extremely low amounts of sample. In peptidomic approaches, CE is commonly combined with mass spectrometric (MS) detection. Most often, CE is coupled with electrospray ionization MS and less frequently with matrix-assisted laser desorption/ionization MS. CE-MS has been employed in numerous studies dealing with determination of peptide biomarkers in different body fluids for various diseases, or in food peptidomic research for the analysis and identification of peptides with special biological activities. In addition to the above topics, sample preparation techniques commonly applied in peptidomics before CE separation and possibilities for peptide identification and quantification by CE-MS or CE-MS/MS methods are discussed in this chapter.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-03899S" target="_blank" >GA20-03899S: Afinitní kapilární elektrokinetické metody pro selektivní analýzu biopolymerů a metabolitů a pro studium jejich interakcí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Separation techniques applied to omics sciences: from principles to relevant applications

ISBN

978-3-030-77251-2

Počet stran výsledku

18

Strana od-do

87-104

Počet stran knihy

273

Název nakladatele

Springer

Místo vydání

Cham

Kód UT WoS kapitoly

000755261400006