Self-Assembly, Drug Encapsulation, and Cellular Uptake of Block and Gradient Copolymers of 2-Methyl-2-oxazine and 2- n-Propyl/butyl-2-oxazoline

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00549833" target="_blank" >RIV/61388963:_____/21:00549833 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/21:00549608 RIV/00216208:11110/21:10437543 RIV/00216208:11320/21:10437543 RIV/00216208:11310/21:10437543

Výsledek na webu

<a href="https://doi.org/10.1021/acs.macromol.1c01794" target="_blank" >https://doi.org/10.1021/acs.macromol.1c01794</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.macromol.1c01794" target="_blank" >10.1021/acs.macromol.1c01794</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Self-Assembly, Drug Encapsulation, and Cellular Uptake of Block and Gradient Copolymers of 2-Methyl-2-oxazine and 2- n-Propyl/butyl-2-oxazoline

Popis výsledku v původním jazyce

Self-assembled amphiphilic polymers have been extensively studied for various biomedical applications, as they show advantageous properties for diagnosis and therapy. In this work, we extensively compared amphiphilic copolymers of the hydrophilic monomer 2-methyl-2-oxazine (MeOzi) and the thermoresponsive or hydrophobic monomers 2-propyl-2-oxazoline (PrOx) or 2-butyl-2-oxazoline (BuOx) in both block and gradient monomer distributions. Such a head-to-head comparison between block and gradient copolymers, which has thus far been mostly missing in the available literature, should provide important insight into the differences and similarities between these two architectures. We investigated the properties of our polymers using a wide array of analytical methods, including dynamic light scattering (DLS), small-angle neutron (SANS) and X-ray scattering (SAXS), one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy, transmission electron microscopy (TEM), drug loading (DL), cellular uptake, and cytotoxicity studies. Most of the studied polymers formed self-assembled nanoparticles, but their properties varied with the monomer ratio, polymer length, and polymer architecture, and these factors could be used to fine-tune the properties of the polymer to meet the demands of the desired application. Both block and gradient copolymers showed similar critical association concentrations and DL properties for the antituberculosis drug rifampicin. Finally, we confirmed that the nanoparticles could be internalized by macrophages, which indicates great potential for the utilization of these nanoparticles in drug delivery.

Název v anglickém jazyce

Self-Assembly, Drug Encapsulation, and Cellular Uptake of Block and Gradient Copolymers of 2-Methyl-2-oxazine and 2- n-Propyl/butyl-2-oxazoline

Popis výsledku anglicky

Self-assembled amphiphilic polymers have been extensively studied for various biomedical applications, as they show advantageous properties for diagnosis and therapy. In this work, we extensively compared amphiphilic copolymers of the hydrophilic monomer 2-methyl-2-oxazine (MeOzi) and the thermoresponsive or hydrophobic monomers 2-propyl-2-oxazoline (PrOx) or 2-butyl-2-oxazoline (BuOx) in both block and gradient monomer distributions. Such a head-to-head comparison between block and gradient copolymers, which has thus far been mostly missing in the available literature, should provide important insight into the differences and similarities between these two architectures. We investigated the properties of our polymers using a wide array of analytical methods, including dynamic light scattering (DLS), small-angle neutron (SANS) and X-ray scattering (SAXS), one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectroscopy, transmission electron microscopy (TEM), drug loading (DL), cellular uptake, and cytotoxicity studies. Most of the studied polymers formed self-assembled nanoparticles, but their properties varied with the monomer ratio, polymer length, and polymer architecture, and these factors could be used to fine-tune the properties of the polymer to meet the demands of the desired application. Both block and gradient copolymers showed similar critical association concentrations and DL properties for the antituberculosis drug rifampicin. Finally, we confirmed that the nanoparticles could be internalized by macrophages, which indicates great potential for the utilization of these nanoparticles in drug delivery.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

<a href="/cs/project/GA19-01602S" target="_blank" >GA19-01602S: Samouspořádané struktury amfifilních gradientových kopolymerů pro koncepčně nové aplikace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Macromolecules

ISSN

0024-9297

e-ISSN

1520-5835

Svazek periodika

54

Číslo periodika v rámci svazku

23

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

10667-10681

Kód UT WoS článku

000752886100004

EID výsledku v databázi Scopus

2-s2.0-85120725708