Optimizing the Chemiluminescence of a Light-Producing Deoxyribozyme

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00556678" target="_blank" >RIV/61388963:_____/22:00556678 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/22:10446953

Výsledek na webu

<a href="https://doi.org/10.1002/cbic.202200026" target="_blank" >https://doi.org/10.1002/cbic.202200026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cbic.202200026" target="_blank" >10.1002/cbic.202200026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimizing the Chemiluminescence of a Light-Producing Deoxyribozyme

Popis výsledku v původním jazyce

Supernova is a chemiluminescent deoxyribozyme recently discovered in our group. It transfers the phosphate group from the 1,2-dioxetane substrate CDP-Star to its 5 ' hydroxyl group, which triggers a decomposition reaction and the production of light. Here we investigated the effects of reaction conditions on the ability of Supernova to generate a chemiluminescent signal (using a plate reader assay) and to phosphorylate itself (using a ligation assay). Our experiments indicate that multiple zinc ions are required for catalytic function, suggesting links between Supernova and protein enzymes that catalyze similar reactions. They also show how factors such as pH, potassium concentration, CDP-Star concentration, and DNA concentration affect the reaction. By combining information from different experiments, the rate enhancement of light production was increased by more than 1000-fold. These results should be useful for applications in which Supernova is used as a sensor.

Název v anglickém jazyce

Optimizing the Chemiluminescence of a Light-Producing Deoxyribozyme

Popis výsledku anglicky

Supernova is a chemiluminescent deoxyribozyme recently discovered in our group. It transfers the phosphate group from the 1,2-dioxetane substrate CDP-Star to its 5 ' hydroxyl group, which triggers a decomposition reaction and the production of light. Here we investigated the effects of reaction conditions on the ability of Supernova to generate a chemiluminescent signal (using a plate reader assay) and to phosphorylate itself (using a ligation assay). Our experiments indicate that multiple zinc ions are required for catalytic function, suggesting links between Supernova and protein enzymes that catalyze similar reactions. They also show how factors such as pH, potassium concentration, CDP-Star concentration, and DNA concentration affect the reaction. By combining information from different experiments, the rate enhancement of light production was increased by more than 1000-fold. These results should be useful for applications in which Supernova is used as a sensor.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA19-20989S" target="_blank" >GA19-20989S: Produkce světla pomocí DNA</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chembiochem

ISSN

1439-4227

e-ISSN

1439-7633

Svazek periodika

23

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

e202200026

Kód UT WoS článku

000775093700001

EID výsledku v databázi Scopus

2-s2.0-85127512464