Visualization of Sentinel Lymph Nodes with Mannosylated Fluorescent Nanodiamonds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00556784" target="_blank" >RIV/61388963:_____/22:00556784 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15640/22:73612541 RIV/61989592:15110/22:73612541 RIV/00216208:11110/22:10442719

Výsledek na webu

<a href="https://doi.org/10.1002/adfm.202109960" target="_blank" >https://doi.org/10.1002/adfm.202109960</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/adfm.202109960" target="_blank" >10.1002/adfm.202109960</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Visualization of Sentinel Lymph Nodes with Mannosylated Fluorescent Nanodiamonds

Popis výsledku v původním jazyce

The present study focuses on the development of a sensitive and specific approach for the visualization of sentinel lymph nodes draining the tumor using ultrabright 200-nm fluorescent nanodiamonds (FNDs) equipped with a designed targeting surface architecture. The FNDs with a narrow size distribution are isolated by differential centrifugation, colloidally stabilized with alkyne-functionalized poly(glycerol) and modified with a polyvalent array of mannose (FND-p-Man). In vitro experiments demonstrate an outstanding increase of the particle internalization by the mannose receptor CD206 (MR) and no significant toxicity. MR involvement is confirmed by blocking ligand binding with mannan and a 15.2 mAb (specific anti-MR) in J774A.1 mouse macrophage cell line. In vivo mouse experiments confirm increased retention of FND-p-Man in sentinel lymph nodes of both healthy and B16 melanoma bearing animals. These results suggest that FND-p-Man has potential as a tracer for lymph node visualization in locoregional perioperative cancer diagnostics and as a tool for endoscopic/robotic fluorescence-guided surgery.

Název v anglickém jazyce

Visualization of Sentinel Lymph Nodes with Mannosylated Fluorescent Nanodiamonds

Popis výsledku anglicky

The present study focuses on the development of a sensitive and specific approach for the visualization of sentinel lymph nodes draining the tumor using ultrabright 200-nm fluorescent nanodiamonds (FNDs) equipped with a designed targeting surface architecture. The FNDs with a narrow size distribution are isolated by differential centrifugation, colloidally stabilized with alkyne-functionalized poly(glycerol) and modified with a polyvalent array of mannose (FND-p-Man). In vitro experiments demonstrate an outstanding increase of the particle internalization by the mannose receptor CD206 (MR) and no significant toxicity. MR involvement is confirmed by blocking ligand binding with mannan and a 15.2 mAb (specific anti-MR) in J774A.1 mouse macrophage cell line. In vivo mouse experiments confirm increased retention of FND-p-Man in sentinel lymph nodes of both healthy and B16 melanoma bearing animals. These results suggest that FND-p-Man has potential as a tracer for lymph node visualization in locoregional perioperative cancer diagnostics and as a tool for endoscopic/robotic fluorescence-guided surgery.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced Functional Materials

ISSN

1616-301X

e-ISSN

1616-3028

Svazek periodika

32

Číslo periodika v rámci svazku

23

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

2109960

Kód UT WoS článku

000763337400001

EID výsledku v databázi Scopus

2-s2.0-85125586919