Arabidopsis thaliana NudiXes have RNA-decapping activity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00569480" target="_blank" >RIV/61388963:_____/23:00569480 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/23:10469613

Výsledek na webu

<a href="https://doi.org/10.1039/D2CB00213B" target="_blank" >https://doi.org/10.1039/D2CB00213B</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d2cb00213b" target="_blank" >10.1039/d2cb00213b</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Arabidopsis thaliana NudiXes have RNA-decapping activity

Popis výsledku v původním jazyce

Recent discoveries of various noncanonical RNA caps, such as dinucleoside polyphosphates (NpnN), coenzyme A (CoA), and nicotinamide adenine dinucleotide (NAD) in all domains of life have led to a revision of views on RNA cap function and metabolism. Enzymes from the NudiX family capable of hydrolyzing a polyphosphate backbone attached to a nucleoside are the strongest candidates for degradation of noncanonically capped RNA. The model plant organism Arabidopsis thaliana encodes as many as 28 NudiX enzymes. For most of them, only in vitro substrates in the form of small molecules are known. In our study, we focused on four A. thaliana NudiX enzymes (AtNUDT6, AtNUDT7, AtNUDT19 and AtNUDT27), and we studied whether these enzymes can cleave RNA capped with Np(n)Ns (Ap(2-5)A, Gp(3-4)G, Ap(3-5)G, m(7)Gp(3)G, m(7)Gp(3)A), CoA, ADP-ribose, or NAD(H). While AtNUDT19 preferred NADH-RNA over other types of capped RNA, AtNUDT6 and AtNUDT7 preferentially cleaved Ap(4)A-RNA. The most powerful decapping enzyme was AtNUDT27, which cleaved almost all types of capped RNA at a tenfold lower concentration than the other enzymes. We also compared cleavage efficiency of each enzyme on free small molecules with RNA capped with corresponding molecules. We found that AtNUDT6 prefers free Ap(4)A, while AtNUDT7 preferentially cleaved Ap(4)A-RNA. These findings show that NudiX enzymes may act as RNA-decapping enzymes in A. thaliana and that other noncanonical RNA caps such as Ap(4)A and NADH should be searched for in plant RNA.

Název v anglickém jazyce

Arabidopsis thaliana NudiXes have RNA-decapping activity

Popis výsledku anglicky

Recent discoveries of various noncanonical RNA caps, such as dinucleoside polyphosphates (NpnN), coenzyme A (CoA), and nicotinamide adenine dinucleotide (NAD) in all domains of life have led to a revision of views on RNA cap function and metabolism. Enzymes from the NudiX family capable of hydrolyzing a polyphosphate backbone attached to a nucleoside are the strongest candidates for degradation of noncanonically capped RNA. The model plant organism Arabidopsis thaliana encodes as many as 28 NudiX enzymes. For most of them, only in vitro substrates in the form of small molecules are known. In our study, we focused on four A. thaliana NudiX enzymes (AtNUDT6, AtNUDT7, AtNUDT19 and AtNUDT27), and we studied whether these enzymes can cleave RNA capped with Np(n)Ns (Ap(2-5)A, Gp(3-4)G, Ap(3-5)G, m(7)Gp(3)G, m(7)Gp(3)A), CoA, ADP-ribose, or NAD(H). While AtNUDT19 preferred NADH-RNA over other types of capped RNA, AtNUDT6 and AtNUDT7 preferentially cleaved Ap(4)A-RNA. The most powerful decapping enzyme was AtNUDT27, which cleaved almost all types of capped RNA at a tenfold lower concentration than the other enzymes. We also compared cleavage efficiency of each enzyme on free small molecules with RNA capped with corresponding molecules. We found that AtNUDT6 prefers free Ap(4)A, while AtNUDT7 preferentially cleaved Ap(4)A-RNA. These findings show that NudiX enzymes may act as RNA-decapping enzymes in A. thaliana and that other noncanonical RNA caps such as Ap(4)A and NADH should be searched for in plant RNA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LL1603" target="_blank" >LL1603: Virální RNA modifikace - Esenciální kroky v chemické evoluci proteinových kofaktorů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RSC Chemical Biology

ISSN

2633-0679

e-ISSN

2633-0679

Svazek periodika

4

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

223-228

Kód UT WoS článku

000915348600001

EID výsledku v databázi Scopus

2-s2.0-85150293104