Inhibitors of SARS-CoV-2, Dengue, and Mpox methyltransferases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00599886" target="_blank" >RIV/61388963:_____/24:00599886 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ccsss.cz/index.php/ccsss/issue/view/48/87" target="_blank" >http://www.ccsss.cz/index.php/ccsss/issue/view/48/87</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Inhibitors of SARS-CoV-2, Dengue, and Mpox methyltransferases

Popis výsledku v původním jazyce

Viral methyltransferases (MTases) are critical enzymes that facilitate the replication and survival of various viruses by modifying their RNA. These modifications are essential for the stability, translation, and evasion of host immune responses. Among these enzymes, the non-structural protein 14 (nsp14) from SARS-CoV-2, the virus responsible for the COVID-19 pandemic, plays a dual role as an exoribonuclease and a MTase. The MTase activity of nsp14 is crucial for the methylation of N7 position of viral RNA cap. Similarly, the NS5 protein from Dengue virus (DENV) is a multifunctional enzyme with both RNA-dependent RNA polymerase and MTase activities. The MTase function of NS5 is responsible for the methylation of the viral RNA cap both at N7 position of the GTP and 2’-O of the first viral RNA nucleotide. This ability to methylate the viral cap at both of these positions is quite specific to Flavivirus NS5 proteins, whereas in both human cells and most other viruses it is mediated by two different proteins/enzymes. In the case of Mpox (formerly known as monkeypox), the VP39 protein serves as a viral 2’-O-methyltransferase. VP39 is involved in the methylation of viral mRNA, which helps the virus to efficiently replicate and evade the host’s immune defenses.

Název v anglickém jazyce

Inhibitors of SARS-CoV-2, Dengue, and Mpox methyltransferases

Popis výsledku anglicky

Viral methyltransferases (MTases) are critical enzymes that facilitate the replication and survival of various viruses by modifying their RNA. These modifications are essential for the stability, translation, and evasion of host immune responses. Among these enzymes, the non-structural protein 14 (nsp14) from SARS-CoV-2, the virus responsible for the COVID-19 pandemic, plays a dual role as an exoribonuclease and a MTase. The MTase activity of nsp14 is crucial for the methylation of N7 position of viral RNA cap. Similarly, the NS5 protein from Dengue virus (DENV) is a multifunctional enzyme with both RNA-dependent RNA polymerase and MTase activities. The MTase function of NS5 is responsible for the methylation of the viral RNA cap both at N7 position of the GTP and 2’-O of the first viral RNA nucleotide. This ability to methylate the viral cap at both of these positions is quite specific to Flavivirus NS5 proteins, whereas in both human cells and most other viruses it is mediated by two different proteins/enzymes. In the case of Mpox (formerly known as monkeypox), the VP39 protein serves as a viral 2’-O-methyltransferase. VP39 is involved in the methylation of viral mRNA, which helps the virus to efficiently replicate and evade the host’s immune defenses.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů