A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00600179" target="_blank" >RIV/61388963:_____/25:00600179 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.bbamem.2024.184388" target="_blank" >https://doi.org/10.1016/j.bbamem.2024.184388</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbamem.2024.184388" target="_blank" >10.1016/j.bbamem.2024.184388</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

Popis výsledku v původním jazyce

Cellular membranes are composed of lipids typically organized in a double-leaflet structure. Interactions between these two leaflets often referred to as interleaflet coupling play a crucial role in various cellular processes. Despite extensive study, the mechanisms governing such interactions remain incompletely understood. Here, we investigate the effects of interleaflet coupling from a specific point of view, i.e. by comparing diffusive dynamics in bilayers and monolayers, focusing on potential lipid-specific interactions between opposing leaflets. Through quantitative fluorescence microscopy techniques, we characterize lipid diffusion and mean molecular area in monolayers and bilayers composed of different lipids. Our results suggest that the observed decrease in bilayer lipid diffusion compared to monolayers depends on lipid identity. Furthermore, our analysis suggests that lipid acyl chain structure and spatial configuration at the bilayer may strongly influence interleaflet interactions and dynamics in bilayers. These findings provide insights into the role of lipid structure in mediating interleaflet coupling and underscore the need for further experimental investigations to elucidate the underlying mechanisms.

Název v anglickém jazyce

A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

Popis výsledku anglicky

Cellular membranes are composed of lipids typically organized in a double-leaflet structure. Interactions between these two leaflets often referred to as interleaflet coupling play a crucial role in various cellular processes. Despite extensive study, the mechanisms governing such interactions remain incompletely understood. Here, we investigate the effects of interleaflet coupling from a specific point of view, i.e. by comparing diffusive dynamics in bilayers and monolayers, focusing on potential lipid-specific interactions between opposing leaflets. Through quantitative fluorescence microscopy techniques, we characterize lipid diffusion and mean molecular area in monolayers and bilayers composed of different lipids. Our results suggest that the observed decrease in bilayer lipid diffusion compared to monolayers depends on lipid identity. Furthermore, our analysis suggests that lipid acyl chain structure and spatial configuration at the bilayer may strongly influence interleaflet interactions and dynamics in bilayers. These findings provide insights into the role of lipid structure in mediating interleaflet coupling and underscore the need for further experimental investigations to elucidate the underlying mechanisms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Biomembranes

ISSN

0005-2736

e-ISSN

1879-2642

Svazek periodika

1867

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

184388

Kód UT WoS článku

001343509500001

EID výsledku v databázi Scopus

2-s2.0-85206948365