Discovery of metabolites prevails amid in-source fragmentation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00618108" target="_blank" >RIV/61388963:_____/25:00618108 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1038/s42255-025-01239-4" target="_blank" >https://doi.org/10.1038/s42255-025-01239-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s42255-025-01239-4" target="_blank" >10.1038/s42255-025-01239-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Discovery of metabolites prevails amid in-source fragmentation

Popis výsledku v původním jazyce

Recent claims suggest that most unannotated signals in untargeted LC–MS metabolomics arise from in-source fragmentation (ISF), implying that the “dark metabolome” may be largely a technical artifact. Here, we argue that this interpretation is overstated and not sufficiently supported by publicly available data. Our analyses indicate that ISFs are typically few and of low intensity relative to precursor ions, and that modern data-processing workflows are capable of identifying and accounting for these ion forms. Furthermore, analysis of a well-characterized human fecal reference sample shows that the majority of detected molecular features remain unannotated even after grouping related ion forms. Together, these findings support the continued existence of a substantial dark metabolome and highlight ongoing opportunities for metabolite discovery.

Název v anglickém jazyce

Discovery of metabolites prevails amid in-source fragmentation

Popis výsledku anglicky

Recent claims suggest that most unannotated signals in untargeted LC–MS metabolomics arise from in-source fragmentation (ISF), implying that the “dark metabolome” may be largely a technical artifact. Here, we argue that this interpretation is overstated and not sufficiently supported by publicly available data. Our analyses indicate that ISFs are typically few and of low intensity relative to precursor ions, and that modern data-processing workflows are capable of identifying and accounting for these ion forms. Furthermore, analysis of a well-characterized human fecal reference sample shows that the majority of detected molecular features remain unannotated even after grouping related ion forms. Together, these findings support the continued existence of a substantial dark metabolome and highlight ongoing opportunities for metabolite discovery.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Metabolism

ISSN

2522-5812

e-ISSN

2522-5812

Svazek periodika

7

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

3

Strana od-do

435-437

Kód UT WoS článku

001434834800001

EID výsledku v databázi Scopus

2-s2.0-85219017556