Mass spectrometry imaging of free-floating sections for the study of Alzheimer’s disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00639573" target="_blank" >RIV/61388963:_____/25:00639573 - isvavai.cz</a>

Výsledek na webu

<a href="https://hdl.handle.net/11104/0369999" target="_blank" >https://hdl.handle.net/11104/0369999</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mass spectrometry imaging of free-floating sections for the study of Alzheimer’s disease

Popis výsledku v původním jazyce

Sample collection and storage are important steps in the mass spectrometry imaging (MSI) sample preparation procedure. The recommended and most widely used procedure for MSI preparation involves freezing the sample in isopentane and storing it at -80 °C. On the other hand, the most common and general method for preserving biological samples in clinical practice is fixation in paraformaldehyde. In the present work, we investigated paraformaldehyde-fixed free-floating sections and studied their applicability for MSI analysis of lipids in the mouse brain. The developed procedure was used in a project aimed at determining lipid changes in double transgenic APP/PS1 mice, a model of Alzheimer’s-like pathology. Moreover, we examined the neuroprotective properties of palm11-PrRP31, an anorexigenic and glucose-lowering analog of prolactin-releasing peptide, using the MSI method. From the free-floating sections, we obtained lipid images without interferences or delocalization. In the APP/PS1 mouse model, we observed altered distribution of various lipids. An increased concentration of gangliosides (GM2 36:1, GM3 36:1) and phosphatidylinositols (PI 38:4, 36:4) and a decreased concentration of sulfatides (ST 18:0, 22:0, 24:0 and ST-OH 22:0, 24:0) were observed in areas where plaque accumulation occurred, compared to the surrounding tissue. In APP/PS1 mice treated with palm11-PrRP31 for 2 months, we found a reduced area of lipids associated with senile plaques. The results indicate that palm11-PrRP31 may potentially be useful in the treatment of neurodegenerative diseases. However, the mechanisms behind its neuroprotective function require further investigation.

Název v anglickém jazyce

Mass spectrometry imaging of free-floating sections for the study of Alzheimer’s disease

Popis výsledku anglicky

Sample collection and storage are important steps in the mass spectrometry imaging (MSI) sample preparation procedure. The recommended and most widely used procedure for MSI preparation involves freezing the sample in isopentane and storing it at -80 °C. On the other hand, the most common and general method for preserving biological samples in clinical practice is fixation in paraformaldehyde. In the present work, we investigated paraformaldehyde-fixed free-floating sections and studied their applicability for MSI analysis of lipids in the mouse brain. The developed procedure was used in a project aimed at determining lipid changes in double transgenic APP/PS1 mice, a model of Alzheimer’s-like pathology. Moreover, we examined the neuroprotective properties of palm11-PrRP31, an anorexigenic and glucose-lowering analog of prolactin-releasing peptide, using the MSI method. From the free-floating sections, we obtained lipid images without interferences or delocalization. In the APP/PS1 mouse model, we observed altered distribution of various lipids. An increased concentration of gangliosides (GM2 36:1, GM3 36:1) and phosphatidylinositols (PI 38:4, 36:4) and a decreased concentration of sulfatides (ST 18:0, 22:0, 24:0 and ST-OH 22:0, 24:0) were observed in areas where plaque accumulation occurred, compared to the surrounding tissue. In APP/PS1 mice treated with palm11-PrRP31 for 2 months, we found a reduced area of lipids associated with senile plaques. The results indicate that palm11-PrRP31 may potentially be useful in the treatment of neurodegenerative diseases. However, the mechanisms behind its neuroprotective function require further investigation.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LX22NPO5104" target="_blank" >LX22NPO5104: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů