Mechanismus rezistence makrolidů a linkosamidů v koaguláze negativních stafylokoků v ČR a nové typy na linkosamid resistentních S. haemolyticus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F04%3A00108129" target="_blank" >RIV/61388971:_____/04:00108129 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Mechanisms of resistance to macrolides and lincosamides in coagulase-negative Staphylococci in the Czech Republic and new type of lincosamide-resistance in S. haemolyticus

Popis výsledku v původním jazyce

Objectives: The aim of this study was to investigate an incidence of different resistance mechanisms to macrolides and lincosamides in methicillin-resistant coagulase-negative staphylococci in the Czech Republic. Methods: Phenotypes were determined by triple-disc diffusion method. The presence of the genes ermA, ermC (resistance by target site alteration) msrA (efflux resistance) and linA (resistance by antibiotic inactivation) was tested by Southern blot analysis. A bioassay for the detection of lincomycin inactivation mechanism was performed in lincosamide-resistant strains, where none of the resistance genes was detected. In these strains also PFGE analysis was done. Results: In 99 clinical isolates in vitro resistant to one of erythromycin, lincomycin or clindamycin, triple-disc diffusion method reveals seven different phenotypes corresponding with resistance mechanisms. The resistance was mainly due to the presence of msrA gene, which was detected in 53 strains. Genes ermC...

Název v anglickém jazyce

Mechanisms of resistance to macrolides and lincosamides in coagulase-negative Staphylococci in the Czech Republic and new type of lincosamide-resistance in S. haemolyticus

Popis výsledku anglicky

Objectives: The aim of this study was to investigate an incidence of different resistance mechanisms to macrolides and lincosamides in methicillin-resistant coagulase-negative staphylococci in the Czech Republic. Methods: Phenotypes were determined by triple-disc diffusion method. The presence of the genes ermA, ermC (resistance by target site alteration) msrA (efflux resistance) and linA (resistance by antibiotic inactivation) was tested by Southern blot analysis. A bioassay for the detection of lincomycin inactivation mechanism was performed in lincosamide-resistant strains, where none of the resistance genes was detected. In these strains also PFGE analysis was done. Results: In 99 clinical isolates in vitro resistant to one of erythromycin, lincomycin or clindamycin, triple-disc diffusion method reveals seven different phenotypes corresponding with resistance mechanisms. The resistance was mainly due to the presence of msrA gene, which was detected in 53 strains. Genes ermC...

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Kongres Československé společnosti mikrobiologické /23./

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

234

Název nakladatele

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Místo vydání

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Místo konání akce

Brno

Datum konání akce

6. 9. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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