Aktivita ribosomů a tmRNA Streptomyces aureofaciens v průběhu vývoje a za stresových podmínek indukovaných změnami teploty a přítomností antibiotik

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F07%3A00095726" target="_blank" >RIV/61388971:_____/07:00095726 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Activity of ribosomes and tmRNA of Streptomyces aureofaciens during development and stress conditions induced by changes in temperature and the presence of antibiotics

Popis výsledku v původním jazyce

Streptomycetes are soil microorganisms exposed to various stresses that activate specialized responses whose coordinated action promotes growth under adverse conditions. Ribosomes having a highly cooperative structure are potential target for control mechanisms that generate signal and activate adaptive regulons or developmental programs. We examined how Streptomyces aureofaciens producing tetracycline responds to the presence of antibiotics and stresses induced by the changes in temperature. Tetracycline interacts with 16S RNA and decreases its thermodynamic stability. The drug also inhibits binding of ternary complex Phe-tRNA.EF-Tu.GTP to purified ribosomes. We have found that antibiotics that cause ribosome stall or pause could increase the requirement for tmRNA in the process trans-translation. Increase in tmRNA level was also demonstrated upon downshift in temperature

Název v anglickém jazyce

Activity of ribosomes and tmRNA of Streptomyces aureofaciens during development and stress conditions induced by changes in temperature and the presence of antibiotics

Popis výsledku anglicky

Streptomycetes are soil microorganisms exposed to various stresses that activate specialized responses whose coordinated action promotes growth under adverse conditions. Ribosomes having a highly cooperative structure are potential target for control mechanisms that generate signal and activate adaptive regulons or developmental programs. We examined how Streptomyces aureofaciens producing tetracycline responds to the presence of antibiotics and stresses induced by the changes in temperature. Tetracycline interacts with 16S RNA and decreases its thermodynamic stability. The drug also inhibits binding of ternary complex Phe-tRNA.EF-Tu.GTP to purified ribosomes. We have found that antibiotics that cause ribosome stall or pause could increase the requirement for tmRNA in the process trans-translation. Increase in tmRNA level was also demonstrated upon downshift in temperature

Druh

C - Kapitola v odborné knize

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F05%2F0106" target="_blank" >GA203/05/0106: Význam trans-translace v buněčné diferenciaci a rezistenci k produkovaným antibiotikům u streptomycet</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Communicating Current Research and Educational Topics

ISBN

978-84-611-9422-3

Počet stran výsledku

7

Strana od-do

129-135

Počet stran knihy

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Název nakladatele

Formatex

Místo vydání

Sevilla

Kód UT WoS kapitoly

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