VDAC2 a aldoláza A identifikovány jako membránové proteiny buněk K562 se zvýšenou expresí za podmínek deprivace železa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F08%3A00308384" target="_blank" >RIV/61388971:_____/08:00308384 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/08:00308384 RIV/00216208:11120/08:00000522

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation

Popis výsledku v původním jazyce

We have shown previously that iron deprivation significantly stimulates the uptake of non-transferrin ferric iron from ferric citrate by erythroleukemia K562cells and that this stimulation depends on protein synthesis. However, we have not detected increased expression of any known iron transport protein. Therefore, in order to identify membrane proteins of K562 cells with increased expression under iron deprivation, we employed the isolation of membrane proteins by two-phase partitioning system, protein separation by two-dimensional electrophoresis, computer differential analysis, and tandem mass spectrometry. Thus, we identified two proteins with statistically significant upregulation, i.e., aldolase A and voltage-dependent anion channel 2 (VDAC2). The upregulation of aldolase A and VDAC2 in K562 cells under iron deprivation was also confirmed by western blot analysis. This is the first time when the control of aldolase A and VDAC2 levels by iron status of the cell is demonstrated

Název v anglickém jazyce

VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation

Popis výsledku anglicky

We have shown previously that iron deprivation significantly stimulates the uptake of non-transferrin ferric iron from ferric citrate by erythroleukemia K562cells and that this stimulation depends on protein synthesis. However, we have not detected increased expression of any known iron transport protein. Therefore, in order to identify membrane proteins of K562 cells with increased expression under iron deprivation, we employed the isolation of membrane proteins by two-phase partitioning system, protein separation by two-dimensional electrophoresis, computer differential analysis, and tandem mass spectrometry. Thus, we identified two proteins with statistically significant upregulation, i.e., aldolase A and voltage-dependent anion channel 2 (VDAC2). The upregulation of aldolase A and VDAC2 in K562 cells under iron deprivation was also confirmed by western blot analysis. This is the first time when the control of aldolase A and VDAC2 levels by iron status of the cell is demonstrated

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA5052402" target="_blank" >IAA5052402: Transport nízkomolekulárního železa do lidských buněk: identifikace transportních molekul</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Biochemistry

ISSN

0300-8177

e-ISSN

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Svazek periodika

311

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

225-231

Kód UT WoS článku

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EID výsledku v databázi Scopus

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