Účast manganové peroxidasy houby Dichomitus squalens v dekolorizaci barviv a spolupráce enzymu s lakasou

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F08%3A00317654" target="_blank" >RIV/61388971:_____/08:00317654 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Implication of Dichomitus squalens manganese-dependent peroxidase in dye decolorization and cooperation of the enzyme with laccase

Popis výsledku v původním jazyce

Three new chromatographical forms of Dichomitus squalens MnP were isolated from wheat-straw cultures using MonoQ and CIM fast protein liquid chromatography. Enzymes revealed identical molecular masses of 50 kDa (estimated by SDS-PAGE) and pI values of 3.50, however, they varied in Km values obtained for Mn2+ oxidation. The addition of wood and straw methanol extracts to D. squalens cultures showed that the production of MnPs in wheat-straw fungal cultures was influenced rather by the type of cultivationthan by phenolic compounds from lignocellulosic material which induced laccase production. The purified CIM1 MnP was able to decolorize selected azo and anthraquinone dyes more rapidly than D. squalens laccase Lc1 per unit of enzyme activity. In vitro dye decolorization also showed a synergistic cooperation of MnP and laccase. In CSB degradation MnP prevented from production of a differently colored product yielded from degradation by laccase/HBT system

Název v anglickém jazyce

Implication of Dichomitus squalens manganese-dependent peroxidase in dye decolorization and cooperation of the enzyme with laccase

Popis výsledku anglicky

Three new chromatographical forms of Dichomitus squalens MnP were isolated from wheat-straw cultures using MonoQ and CIM fast protein liquid chromatography. Enzymes revealed identical molecular masses of 50 kDa (estimated by SDS-PAGE) and pI values of 3.50, however, they varied in Km values obtained for Mn2+ oxidation. The addition of wood and straw methanol extracts to D. squalens cultures showed that the production of MnPs in wheat-straw fungal cultures was influenced rather by the type of cultivationthan by phenolic compounds from lignocellulosic material which induced laccase production. The purified CIM1 MnP was able to decolorize selected azo and anthraquinone dyes more rapidly than D. squalens laccase Lc1 per unit of enzyme activity. In vitro dye decolorization also showed a synergistic cooperation of MnP and laccase. In CSB degradation MnP prevented from production of a differently colored product yielded from degradation by laccase/HBT system

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Svazek periodika

53

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000262754600003

EID výsledku v databázi Scopus

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