Fast estimation of modes of action of antimicrobials on yeast Saccharomyces cerevisiae - from MDR pump inhibition with a minimal effect on membrane potential up to cell permeabilizatio

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F09%3A00340980" target="_blank" >RIV/61388971:_____/09:00340980 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fast estimation of modes of action of antimicrobials on yeast Saccharomyces cerevisiae - from MDR pump inhibition with a minimal effect on membrane potential up to cell permeabilizatio

Popis výsledku v původním jazyce

Antimicrobial drugs that target directly the cell surface structures, resulting in cell permeabilization, were screened by our fluorescence method based on the use of the potentiometric fluorescent probe diS-C3(3). DiS-C3(3) is a substrate for two majorS. cerevisiae MDR pumps, Pdr5p and Snq2p. Its use represents an efficient strategy for identifying pump inhibitors with minimal side effects on membrane potential or membrane integrity, and for detecting cells permeabilized by surface active drugs. The screening is performed on cells in well-defined physiological state and does not require any pretreatment or preincubation

Název v anglickém jazyce

Fast estimation of modes of action of antimicrobials on yeast Saccharomyces cerevisiae - from MDR pump inhibition with a minimal effect on membrane potential up to cell permeabilizatio

Popis výsledku anglicky

Antimicrobial drugs that target directly the cell surface structures, resulting in cell permeabilization, were screened by our fluorescence method based on the use of the potentiometric fluorescent probe diS-C3(3). DiS-C3(3) is a substrate for two majorS. cerevisiae MDR pumps, Pdr5p and Snq2p. Its use represents an efficient strategy for identifying pump inhibitors with minimal side effects on membrane potential or membrane integrity, and for detecting cells permeabilized by surface active drugs. The screening is performed on cells in well-defined physiological state and does not require any pretreatment or preincubation

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/1M0570" target="_blank" >1M0570: Výzkumné centrum pro studium obsahových látek ječmene a chmele</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů