Antibody repertoire development in fetal and neonatal piglets. XXII. lambda rearrangement precedes kappa rearrangement during B-cell lymphogenesis in swine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00386940" target="_blank" >RIV/61388971:_____/12:00386940 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1365-2567.2012.03615.x" target="_blank" >http://dx.doi.org/10.1111/j.1365-2567.2012.03615.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1365-2567.2012.03615.x" target="_blank" >10.1111/j.1365-2567.2012.03615.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antibody repertoire development in fetal and neonatal piglets. XXII. lambda rearrangement precedes kappa rearrangement during B-cell lymphogenesis in swine

Popis výsledku v původním jazyce

VDJ and VJ rearrangements, expression of RAG-1, Tdt and VpreB, and the presence of signal joint circles (SJC) were used to identify sites of B-cell lymphogenesis. VDJ, V?J? but not V?J? rearrangements or SJC were recovered from yolk sac (YS) at 20 similar to days of gestation (DG) along with strong expression of VpreB and RAG-1 but weak Tdt expression. V?J? rearrangements but not V?J? rearrangements were recovered from fetal liver at 3050 DG. SJC were pronounced in bone marrow at 95 DG where V?J? rearrangements were first recovered. The V?J? rearrangements recovered at 2050 DG used some of the same V? and J? segments seen in older fetuses and adult animals. Hence the textbook paradigm for the order of light-chain rearrangement does not apply to swine.Consistent with weak Tdt expression in early sites of lymphogenesis, N-region additions in VDJ rearrangements were more frequent at 95 DG. Junctional diversity in V?J? rearrangement was limited at all stages of development. There was litt

Název v anglickém jazyce

Antibody repertoire development in fetal and neonatal piglets. XXII. lambda rearrangement precedes kappa rearrangement during B-cell lymphogenesis in swine

Popis výsledku anglicky

VDJ and VJ rearrangements, expression of RAG-1, Tdt and VpreB, and the presence of signal joint circles (SJC) were used to identify sites of B-cell lymphogenesis. VDJ, V?J? but not V?J? rearrangements or SJC were recovered from yolk sac (YS) at 20 similar to days of gestation (DG) along with strong expression of VpreB and RAG-1 but weak Tdt expression. V?J? rearrangements but not V?J? rearrangements were recovered from fetal liver at 3050 DG. SJC were pronounced in bone marrow at 95 DG where V?J? rearrangements were first recovered. The V?J? rearrangements recovered at 2050 DG used some of the same V? and J? segments seen in older fetuses and adult animals. Hence the textbook paradigm for the order of light-chain rearrangement does not apply to swine.Consistent with weak Tdt expression in early sites of lymphogenesis, N-region additions in VDJ rearrangements were more frequent at 95 DG. Junctional diversity in V?J? rearrangement was limited at all stages of development. There was litt

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Immunology

ISSN

0019-2805

e-ISSN

—

Svazek periodika

137

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

149-159

Kód UT WoS článku

000308405700005

EID výsledku v databázi Scopus

—