Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00376216" target="_blank" >RIV/61388971:_____/13:00376216 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985807:_____/13:00376216 RIV/60076658:12520/13:43885239 RIV/61388971:_____/13:00425117

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.mcm.2011.12.029" target="_blank" >http://dx.doi.org/10.1016/j.mcm.2011.12.029</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mcm.2011.12.029" target="_blank" >10.1016/j.mcm.2011.12.029</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images

Popis výsledku v původním jazyce

The determination of phycobilisome diffusivity (diffusion coefficient D) on thylakoid membrane from fluorescence recovery after photobleaching (FRAP) experiments is usually done by analytical models. However, analytical models need some unrealistic conditions to be supposed. This study describes the development of a method based on finite difference approximation of the process governed by the Fickian diffusion equation and on the minimization of an objective function representing the disparity betweenthe experimental and simulated time-varying concentration profiles. Our method improves on other models by accounting for experimentally measured time-varying Dirichlet boundary conditions, and can include a reaction term as well. As a result we obtain both the overall (time averaged) diffusion coefficient D and the sequence of diffusivities Dj based on two successive fluorescence profiles in j-th time interval. Due to the noisy data, we cope with an inverse ill-posed problem and a regul

Název v anglickém jazyce

Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images

Popis výsledku anglicky

The determination of phycobilisome diffusivity (diffusion coefficient D) on thylakoid membrane from fluorescence recovery after photobleaching (FRAP) experiments is usually done by analytical models. However, analytical models need some unrealistic conditions to be supposed. This study describes the development of a method based on finite difference approximation of the process governed by the Fickian diffusion equation and on the minimization of an objective function representing the disparity betweenthe experimental and simulated time-varying concentration profiles. Our method improves on other models by accounting for experimentally measured time-varying Dirichlet boundary conditions, and can include a reaction term as well. As a result we obtain both the overall (time averaged) diffusion coefficient D and the sequence of diffusivities Dj based on two successive fluorescence profiles in j-th time interval. Due to the noisy data, we cope with an inverse ill-posed problem and a regul

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BA - Obecná matematika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mathematical and Computer Modelling

ISSN

0895-7177

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

7-8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

1907-1912

Kód UT WoS článku

000315863300045

EID výsledku v databázi Scopus

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