Determination of antibiotics in influents and effluents of wastewater-treatment-plants in the Czech Republic ? development and application of the SPE and a UHPLC-ToFMS method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00395780" target="_blank" >RIV/61388971:_____/13:00395780 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60193697:_____/13:#0000831

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c3ay00048f" target="_blank" >http://dx.doi.org/10.1039/c3ay00048f</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c3ay00048f" target="_blank" >10.1039/c3ay00048f</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of antibiotics in influents and effluents of wastewater-treatment-plants in the Czech Republic ? development and application of the SPE and a UHPLC-ToFMS method

Popis výsledku v původním jazyce

A method consisting of solid phase extraction followed by ultra-high performance liquid chromatography combined with time-of-flight mass spectrometry (UHPLC-ToFMS) has been developed for the determination of multiple-class antibiotics in influents and effluents of wastewater-treatment-plants (WWTPs). The nineteen analyzed antibiotics belong to the 5 most prescribed antibiotic classes in the Czech Republic, namely tetracyclines, macrolides, sulfonamides, fluoroquinolones, and lincosamides. The matrix-matched calibration technique with internal standard addition for each antibiotic group was employed for quantification. Two extractions employing an Oasis HLB cartridge and two injections were needed for each sample due to the different physico-chemical properties of the tested antibiotics. Prior to the extraction on the Oasis HLB SPE cartridge, the samples were adjusted to pH 4.5 and 7.5. The usefulness and versatility of the method was documented by achieving method limits of quantificat

Název v anglickém jazyce

Determination of antibiotics in influents and effluents of wastewater-treatment-plants in the Czech Republic ? development and application of the SPE and a UHPLC-ToFMS method

Popis výsledku anglicky

A method consisting of solid phase extraction followed by ultra-high performance liquid chromatography combined with time-of-flight mass spectrometry (UHPLC-ToFMS) has been developed for the determination of multiple-class antibiotics in influents and effluents of wastewater-treatment-plants (WWTPs). The nineteen analyzed antibiotics belong to the 5 most prescribed antibiotic classes in the Czech Republic, namely tetracyclines, macrolides, sulfonamides, fluoroquinolones, and lincosamides. The matrix-matched calibration technique with internal standard addition for each antibiotic group was employed for quantification. Two extractions employing an Oasis HLB cartridge and two injections were needed for each sample due to the different physico-chemical properties of the tested antibiotics. Prior to the extraction on the Oasis HLB SPE cartridge, the samples were adjusted to pH 4.5 and 7.5. The usefulness and versatility of the method was documented by achieving method limits of quantificat

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/1M06011" target="_blank" >1M06011: Centrum molekulárních metod monitorování difúzního znečištění životního prostředí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods: advancing methods and applications

ISSN

1759-9660

e-ISSN

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Svazek periodika

5

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

2110-2118

Kód UT WoS článku

000316960200031

EID výsledku v databázi Scopus

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