The RNA Chaperone Hfq Is Required for Virulence of Bordetella pertussis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00399902" target="_blank" >RIV/61388971:_____/13:00399902 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1128/IAI.00345-13" target="_blank" >http://dx.doi.org/10.1128/IAI.00345-13</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/IAI.00345-13" target="_blank" >10.1128/IAI.00345-13</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The RNA Chaperone Hfq Is Required for Virulence of Bordetella pertussis

Popis výsledku v původním jazyce

Bordetella pertussis is a Gram-negative pathogen causing the human respiratory disease called pertussis or whooping cough. Here we examined the role of the RNA chaperone Hfq in B. pertussis virulence. Hfq mediates interactions between small regulatory RNAs and their mRNA targets and thus plays an important role in posttranscriptional regulation of many cellular processes in bacteria, including production of virulence factors. We characterized an hfq deletion mutant (Delta hfq) of B. pertussis 18323 andshow that the Delta hfq strain produces decreased amounts of the adenylate cyclase toxin that plays a central role in B. pertussis virulence. Production of pertussis toxin and filamentous hemagglutinin was affected to a lesser extent. In vitro, the ability of the Delta hfq strain to survive within macrophages was significantly reduced compared to that of the wild-type (wt) strain. The virulence of the Delta hfq strain in the mouse respiratory model of infection was attenuated, with its c

Název v anglickém jazyce

The RNA Chaperone Hfq Is Required for Virulence of Bordetella pertussis

Popis výsledku anglicky

Bordetella pertussis is a Gram-negative pathogen causing the human respiratory disease called pertussis or whooping cough. Here we examined the role of the RNA chaperone Hfq in B. pertussis virulence. Hfq mediates interactions between small regulatory RNAs and their mRNA targets and thus plays an important role in posttranscriptional regulation of many cellular processes in bacteria, including production of virulence factors. We characterized an hfq deletion mutant (Delta hfq) of B. pertussis 18323 andshow that the Delta hfq strain produces decreased amounts of the adenylate cyclase toxin that plays a central role in B. pertussis virulence. Production of pertussis toxin and filamentous hemagglutinin was affected to a lesser extent. In vitro, the ability of the Delta hfq strain to survive within macrophages was significantly reduced compared to that of the wild-type (wt) strain. The virulence of the Delta hfq strain in the mouse respiratory model of infection was attenuated, with its c

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Infection and Immunity

ISSN

0019-9567

e-ISSN

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Svazek periodika

81

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

4081-4090

Kód UT WoS článku

000325719900013

EID výsledku v databázi Scopus

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