Induction of protective immunity against Mycobacterium tuberculosis by delivery of ESX antigens into airway dendritic cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00422897" target="_blank" >RIV/61388971:_____/13:00422897 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/mi.2012.92" target="_blank" >http://dx.doi.org/10.1038/mi.2012.92</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/mi.2012.92" target="_blank" >10.1038/mi.2012.92</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Induction of protective immunity against Mycobacterium tuberculosis by delivery of ESX antigens into airway dendritic cells

Popis výsledku v původním jazyce

As the Bacillus Calmette-Guerin (BCG) vaccine does not confer long-lasting protection against lung Mycobacterium tuberculosis infection, the development of more efficient vaccines is greatly needed. Here, we used mycobacterial low-molecular weight proteins of the 6-kDa Early Secreted Antigenic Target (ESAT-6) protein family (ESX) antigens for the evaluation of a novel vaccine delivery strategy that enables versatile in vivo targeting of antigens into specialized dendritic cell (DC) subsets. ESX antigenswere genetically fused to the tetramerizing core of streptavidin (SA) to form high-affinity complexes with biotin (biot)-conjugated antibodies recognizing DC surface receptors. When directed through the CD11b or CD11c beta(2)-integrins or diverse C-typelectins, the ESX-SA:biot-antibody complexes were efficiently captured and presented on major histocompatibility complex molecules of DCs to specific T-cell receptors. Robust ESX-specific T-cell responses were induced by immunization with

Název v anglickém jazyce

Induction of protective immunity against Mycobacterium tuberculosis by delivery of ESX antigens into airway dendritic cells

Popis výsledku anglicky

As the Bacillus Calmette-Guerin (BCG) vaccine does not confer long-lasting protection against lung Mycobacterium tuberculosis infection, the development of more efficient vaccines is greatly needed. Here, we used mycobacterial low-molecular weight proteins of the 6-kDa Early Secreted Antigenic Target (ESAT-6) protein family (ESX) antigens for the evaluation of a novel vaccine delivery strategy that enables versatile in vivo targeting of antigens into specialized dendritic cell (DC) subsets. ESX antigenswere genetically fused to the tetramerizing core of streptavidin (SA) to form high-affinity complexes with biotin (biot)-conjugated antibodies recognizing DC surface receptors. When directed through the CD11b or CD11c beta(2)-integrins or diverse C-typelectins, the ESX-SA:biot-antibody complexes were efficiently captured and presented on major histocompatibility complex molecules of DCs to specific T-cell receptors. Robust ESX-specific T-cell responses were induced by immunization with

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/KAN200520702" target="_blank" >KAN200520702: Nanoimunosenzory pro detekci cytokinů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mucosal Immunology

ISSN

1933-0219

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

522-534

Kód UT WoS článku

000317722800009

EID výsledku v databázi Scopus

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