Plasmodium berghei sporozoite challenge of vaccinated BALB/c mice leads to the induction of humoral immunity and improved function of CD8(+) memory T cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00422913" target="_blank" >RIV/61388971:_____/13:00422913 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/eji.201142262" target="_blank" >http://dx.doi.org/10.1002/eji.201142262</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/eji.201142262" target="_blank" >10.1002/eji.201142262</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Plasmodium berghei sporozoite challenge of vaccinated BALB/c mice leads to the induction of humoral immunity and improved function of CD8(+) memory T cells

Popis výsledku v původním jazyce

Protection against malaria can be achieved by induction of a strong CD8+ T-cell response against the Plasmodium circumsporozoite protein (CSP), but most subunit vaccines suffer from insufficient memory responses. In the present study, we analyzed the impact of postimmunization sporozoite challenge on the development of long-lasting immunity. BALB/c mice were immunized by a heterologous prime/boost regimen against Plasmodium berghei CSP that induces a strong CD8+ T-cell response and sterile protection, which is short-lived. Here, we show that protective immunity is prolonged by a sporozoite challenge after immunization. Repeated challenges induced sporozoite-specific antibodies that showed protective capacity. The numbers of CSP-specific CD8+ Tcells were not substantially enhanced by sporozoite infections; however, CSP-specific memory CD8+ Tcells of challenged mice displayed a higher cytotoxic activity than memory Tcells of immunized-only mice. CD4+ Tcells contributed to protection as w

Název v anglickém jazyce

Plasmodium berghei sporozoite challenge of vaccinated BALB/c mice leads to the induction of humoral immunity and improved function of CD8(+) memory T cells

Popis výsledku anglicky

Protection against malaria can be achieved by induction of a strong CD8+ T-cell response against the Plasmodium circumsporozoite protein (CSP), but most subunit vaccines suffer from insufficient memory responses. In the present study, we analyzed the impact of postimmunization sporozoite challenge on the development of long-lasting immunity. BALB/c mice were immunized by a heterologous prime/boost regimen against Plasmodium berghei CSP that induces a strong CD8+ T-cell response and sterile protection, which is short-lived. Here, we show that protective immunity is prolonged by a sporozoite challenge after immunization. Repeated challenges induced sporozoite-specific antibodies that showed protective capacity. The numbers of CSP-specific CD8+ Tcells were not substantially enhanced by sporozoite infections; however, CSP-specific memory CD8+ Tcells of challenged mice displayed a higher cytotoxic activity than memory Tcells of immunized-only mice. CD4+ Tcells contributed to protection as w

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA310%2F08%2F0447" target="_blank" >GA310/08/0447: Mechanismy jimiž cAMP moduluje funkce buněk imunitního systému</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Immunology

ISSN

0014-2980

e-ISSN

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Svazek periodika

43

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

693-704

Kód UT WoS článku

000316140600015

EID výsledku v databázi Scopus

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