Bacterial communities in tetrachloroethene-polluted groundwaters: A case study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00422916" target="_blank" >RIV/61388971:_____/13:00422916 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.scitotenv.2013.02.082" target="_blank" >http://dx.doi.org/10.1016/j.scitotenv.2013.02.082</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scitotenv.2013.02.082" target="_blank" >10.1016/j.scitotenv.2013.02.082</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bacterial communities in tetrachloroethene-polluted groundwaters: A case study

Popis výsledku v původním jazyce

The compositions of bacterial groundwater communities of three sites contaminated with chlorinated ethenes were analyzed by pyrosequencing their 16S rRNA genes. For each location, the entire and the active bacterial populations were characterized by independent molecular analysis of the community DNA and RNA The sites were selected to cover a broad range of different environmental conditions and contamination levels, with tetrachloroethene (PCE) and trichloroethene (TCE) being the primary contaminants.Before sampling the biomass, a long-term monitoring of the polluted locations revealed high concentrations of cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC), which are toxic by-products of the incomplete bacterial degradation of PCE and TCE. The applied pyrosequencing technique enabled known dechlorinators to be identified at a very low detection level (<0.25%) without compromising the detailed analysis of the entire bacterial community of these sites. The study revealed that only

Název v anglickém jazyce

Bacterial communities in tetrachloroethene-polluted groundwaters: A case study

Popis výsledku anglicky

The compositions of bacterial groundwater communities of three sites contaminated with chlorinated ethenes were analyzed by pyrosequencing their 16S rRNA genes. For each location, the entire and the active bacterial populations were characterized by independent molecular analysis of the community DNA and RNA The sites were selected to cover a broad range of different environmental conditions and contamination levels, with tetrachloroethene (PCE) and trichloroethene (TCE) being the primary contaminants.Before sampling the biomass, a long-term monitoring of the polluted locations revealed high concentrations of cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC), which are toxic by-products of the incomplete bacterial degradation of PCE and TCE. The applied pyrosequencing technique enabled known dechlorinators to be identified at a very low detection level (<0.25%) without compromising the detailed analysis of the entire bacterial community of these sites. The study revealed that only

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP504%2F10%2F0137" target="_blank" >GAP504/10/0137: Analýza mikrobiálních komunit z různých vodních prostředí: specifická transkriptomika ve vztahu k znečištění způsobenému těkavými organickými látkami</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Science of the Total Environment

ISSN

0048-9697

e-ISSN

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Svazek periodika

454

Číslo periodika v rámci svazku

JUN 2013

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

517-527

Kód UT WoS článku

000319180000055

EID výsledku v databázi Scopus

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