Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00423807" target="_blank" >RIV/61388971:_____/13:00423807 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12010-012-9952-9" target="_blank" >http://dx.doi.org/10.1007/s12010-012-9952-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12010-012-9952-9" target="_blank" >10.1007/s12010-012-9952-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase

Popis výsledku v původním jazyce

Production of the lignocellulose-degrading enzymes endo-1,4-beta-glucanase, 1,4-beta-glucosidase, cellobiohydrolase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase, Mn peroxidase, and laccase was characterized in a common wood-rotting fungus Fomes fomentarius, a species able to efficiently decompose dead wood, and compared to the production in eight other fungal species. The main aim of this study was to characterize the 1,4-beta-glucosidase produced by F. fomentarius that was produced in high quantities in liquid stationary culture (25.9 U ml(-1)), at least threefold compared to other saprotrophic basidiomycetes, such as Rhodocollybia butyracea, Hypholoma fasciculare, Irpex lacteus, Fomitopsis pinicola, Pleurotus ostreatus, Piptoporus betulinus, and Gymnopus sp. (between 0.7 and 7.9 U ml(-1)). The 1,4-beta-glucosidase enzyme was purified to electrophoretic homogeneity by both anion-exchange and size-exclusion chromatography

Název v anglickém jazyce

Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase

Popis výsledku anglicky

Production of the lignocellulose-degrading enzymes endo-1,4-beta-glucanase, 1,4-beta-glucosidase, cellobiohydrolase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase, Mn peroxidase, and laccase was characterized in a common wood-rotting fungus Fomes fomentarius, a species able to efficiently decompose dead wood, and compared to the production in eight other fungal species. The main aim of this study was to characterize the 1,4-beta-glucosidase produced by F. fomentarius that was produced in high quantities in liquid stationary culture (25.9 U ml(-1)), at least threefold compared to other saprotrophic basidiomycetes, such as Rhodocollybia butyracea, Hypholoma fasciculare, Irpex lacteus, Fomitopsis pinicola, Pleurotus ostreatus, Piptoporus betulinus, and Gymnopus sp. (between 0.7 and 7.9 U ml(-1)). The 1,4-beta-glucosidase enzyme was purified to electrophoretic homogeneity by both anion-exchange and size-exclusion chromatography

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Biochemistry and Biotechnology

ISSN

0273-2289

e-ISSN

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Svazek periodika

169

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

100-109

Kód UT WoS článku

000314023100009

EID výsledku v databázi Scopus

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