Intracellular sequestration of zinc, cadmium and silver in Hebeloma mesophaeum and characterization of its metallothionein genes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00429961" target="_blank" >RIV/61388971:_____/14:00429961 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389005:_____/14:00429961 RIV/60461373:22330/14:43897719

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.fgb.2014.03.003" target="_blank" >http://dx.doi.org/10.1016/j.fgb.2014.03.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fgb.2014.03.003" target="_blank" >10.1016/j.fgb.2014.03.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Intracellular sequestration of zinc, cadmium and silver in Hebeloma mesophaeum and characterization of its metallothionein genes

Popis výsledku v původním jazyce

Sequestration of intracellular heavy metals in eukaryotes involves compartmentalization and binding with cytosolic, cysteine-rich metallothionein (MT) peptides. We examined the roles of these processes in handling of zinc (Zn), cadmium (Cd) and silver (Ag) in sporocarps and a metal-exposed extraradical mycelium of Hebeloma mesophaeum, the Zn-accumulating ectomycorrhizal (EM) species frequently associated with metal disturbed sites. Size exclusion chromatography revealed that the majority of Zn and Cd inthe sporocarps and mycelium was contained in a low molecular mass fraction attributable to compartmentalized metal. The staining of hyphal cells with the Zn-specific Zinquin and Cd-specific Leadmium fluorescent tracers labeled Zn and Cd in small, punctuated vesicles and vacuoles, respectively. By contrast, the sporocarp and mycelium Ag was associated with cysteine-rich, 5-kDa peptides. The peptides of the same size were also identified in minor Zn and Cd complexes from the metal-exposed

Název v anglickém jazyce

Intracellular sequestration of zinc, cadmium and silver in Hebeloma mesophaeum and characterization of its metallothionein genes

Popis výsledku anglicky

Sequestration of intracellular heavy metals in eukaryotes involves compartmentalization and binding with cytosolic, cysteine-rich metallothionein (MT) peptides. We examined the roles of these processes in handling of zinc (Zn), cadmium (Cd) and silver (Ag) in sporocarps and a metal-exposed extraradical mycelium of Hebeloma mesophaeum, the Zn-accumulating ectomycorrhizal (EM) species frequently associated with metal disturbed sites. Size exclusion chromatography revealed that the majority of Zn and Cd inthe sporocarps and mycelium was contained in a low molecular mass fraction attributable to compartmentalized metal. The staining of hyphal cells with the Zn-specific Zinquin and Cd-specific Leadmium fluorescent tracers labeled Zn and Cd in small, punctuated vesicles and vacuoles, respectively. By contrast, the sporocarp and mycelium Ag was associated with cysteine-rich, 5-kDa peptides. The peptides of the same size were also identified in minor Zn and Cd complexes from the metal-exposed

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP504%2F11%2F0484" target="_blank" >GAP504/11/0484: Hyperakumulace těžkých kovů velkými houbami – molekulární, geomykologické a ekotaxonomické aspekty</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fungal Genetics and Biology

ISSN

1087-1845

e-ISSN

—

Svazek periodika

67

Číslo periodika v rámci svazku

JUN

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

3-14

Kód UT WoS článku

000336562700002

EID výsledku v databázi Scopus

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