Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00435787" target="_blank" >RIV/61388971:_____/14:00435787 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/14:43897784 RIV/60461373:22340/14:43897784

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.mimet.2014.01.008" target="_blank" >http://dx.doi.org/10.1016/j.mimet.2014.01.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mimet.2014.01.008" target="_blank" >10.1016/j.mimet.2014.01.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis

Popis výsledku v původním jazyce

Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI massspectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species

Název v anglickém jazyce

Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis

Popis výsledku anglicky

Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI massspectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP503%2F10%2F0664" target="_blank" >GAP503/10/0664: Isolace, typisace a vývoj imunochemických a instrumentálních metod detekce a charakterisace Cronobacter sp.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiological Methods

ISSN

0167-7012

e-ISSN

—

Svazek periodika

98

Číslo periodika v rámci svazku

MAR 2014

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

105-113

Kód UT WoS článku

000333852700019

EID výsledku v databázi Scopus

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