Preservation of basidiomycete strains on perlite using different protocols

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00435964" target="_blank" >RIV/61388971:_____/14:00435964 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.myc.2014.01.006" target="_blank" >http://dx.doi.org/10.1016/j.myc.2014.01.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.myc.2014.01.006" target="_blank" >10.1016/j.myc.2014.01.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preservation of basidiomycete strains on perlite using different protocols

Popis výsledku v původním jazyce

For preservation of 31 basidiomycete strains on perlite in cryovials we used five different perlite protocols to compare their applicability in laboratories with different equipment, namely a viability of the controlled freezing device or the electric deep-freezer and liquid nitrogen supply. The viability of the strains, macromorphological characteristics and the production of laccase were tested after 48 h, six months and one year of storage in the respective device. Our results indicated that the different response to the freezing/thawing process is an intrinsic feature of the respective strain. Nevertheless, the highest viability and preservation of laccase production in our tested strains was found when we used prefreezing to -80 degrees Cat a freezing rate of 1 degrees C/min in a programmable IceCube 1800 freezer or in freezing container Mr. Frosty before storage in liquid nitrogen or at ultra-low temperature freezer at -80 degrees C, respectively. The two abovementioned protocols

Název v anglickém jazyce

Preservation of basidiomycete strains on perlite using different protocols

Popis výsledku anglicky

For preservation of 31 basidiomycete strains on perlite in cryovials we used five different perlite protocols to compare their applicability in laboratories with different equipment, namely a viability of the controlled freezing device or the electric deep-freezer and liquid nitrogen supply. The viability of the strains, macromorphological characteristics and the production of laccase were tested after 48 h, six months and one year of storage in the respective device. Our results indicated that the different response to the freezing/thawing process is an intrinsic feature of the respective strain. Nevertheless, the highest viability and preservation of laccase production in our tested strains was found when we used prefreezing to -80 degrees Cat a freezing rate of 1 degrees C/min in a programmable IceCube 1800 freezer or in freezing container Mr. Frosty before storage in liquid nitrogen or at ultra-low temperature freezer at -80 degrees C, respectively. The two abovementioned protocols

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP504%2F12%2F0709" target="_blank" >GAP504/12/0709: Biodiverzita basidiomycetů a jejich význam v dekompozičních procesech v prostředí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mycoscience

ISSN

1340-3540

e-ISSN

—

Svazek periodika

55

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

JP - Japonsko

Počet stran výsledku

10

Strana od-do

439-448

Kód UT WoS článku

000342076300004

EID výsledku v databázi Scopus

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