Microscopic fungi isolated from cave air and sediments in the Nerja Cave-preliminary results

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00506893" target="_blank" >RIV/61388971:_____/14:00506893 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Microscopic fungi isolated from cave air and sediments in the Nerja Cave-preliminary results

Popis výsledku v původním jazyce

Previous studies on the fungal community in the Nerja Cave (Malaga, Andalusia) were focused only on a capture of fungal spores from the cave atmosphere and outdoor air by the Hirst-type volumetric pollen traps, which means without cultivation methods. The results of these studies showed the presence of different spore types belonging to Oomycota, Myxomycota, but mainly to Ascomycota and Basidiomycota including their anamorph states, where spores of Penicillium/Aspergillus were dominant. Our study was targeted on cultivable microscopic fungi. Several sites inside the cave, mainly in the Tourist Gallery (2011 and 2012) but also in the Upper Gallery (2012) were chosen and several soil samples were collected above the cave, the isolation from outside air was also used to compare with cave environment. Two isolation methods were used for this study-the gravity settling technique for the isolation of airborne microfungi inside and outside the cave and the dilution plate method for the isolation of microfungi from cave sediment and soil above the cave. The CFU counts of airborne cultivable microfungi were found to be much lower in comparison with previous published data from volumetric traps. Nevertheless a rich microfungal spectrum was isolated from the cave air as well as from the cave sediments. To date, 16 and 6 species of the genus Aspergillus have been identified from cave sediments and cave air, respectively, including one novel species.

Název v anglickém jazyce

Microscopic fungi isolated from cave air and sediments in the Nerja Cave-preliminary results

Popis výsledku anglicky

Previous studies on the fungal community in the Nerja Cave (Malaga, Andalusia) were focused only on a capture of fungal spores from the cave atmosphere and outdoor air by the Hirst-type volumetric pollen traps, which means without cultivation methods. The results of these studies showed the presence of different spore types belonging to Oomycota, Myxomycota, but mainly to Ascomycota and Basidiomycota including their anamorph states, where spores of Penicillium/Aspergillus were dominant. Our study was targeted on cultivable microscopic fungi. Several sites inside the cave, mainly in the Tourist Gallery (2011 and 2012) but also in the Upper Gallery (2012) were chosen and several soil samples were collected above the cave, the isolation from outside air was also used to compare with cave environment. Two isolation methods were used for this study-the gravity settling technique for the isolation of airborne microfungi inside and outside the cave and the dilution plate method for the isolation of microfungi from cave sediment and soil above the cave. The CFU counts of airborne cultivable microfungi were found to be much lower in comparison with previous published data from volumetric traps. Nevertheless a rich microfungal spectrum was isolated from the cave air as well as from the cave sediments. To date, 16 and 6 species of the genus Aspergillus have been identified from cave sediments and cave air, respectively, including one novel species.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Conservation of Subterranean Cultural Heritage

ISBN

978-1-315-73997-7

Počet stran výsledku

7

Strana od-do

239-245

Počet stran knihy

320

Název nakladatele

CRC PRESS-TAYLOR & FRANCIS GROUP

Místo vydání

BOCA RATON, FL

Kód UT WoS kapitoly

000352104800028