Upscale of recombinant alpha-L-rhamnosidase production by Pichia pastoris Mut(S) strain

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00450837" target="_blank" >RIV/61388971:_____/15:00450837 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3389/fmicb.2015.01140" target="_blank" >http://dx.doi.org/10.3389/fmicb.2015.01140</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2015.01140" target="_blank" >10.3389/fmicb.2015.01140</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Upscale of recombinant alpha-L-rhamnosidase production by Pichia pastoris Mut(S) strain

Popis výsledku v původním jazyce

Pichia pastoris is currently one of the most preferred microorganisms for recombinant enzyme production due to its efficient expression system. The advantages include the production of high amounts of recombinant proteins containing the appropriate posttranslational modifications and easy cultivation conditions. alpha-L-Rhamnosidase is a biotechnologically important enzyme in food and pharmaceutical industry, used for example in debittering of citrus fruit juices, rhamnose pruning from naringin, or enhancement of wine aromas, creating a demand for the production of an active and stable enzyme. The production of recombinant alpha-L-rhamnosidase cloned in the Mut(S) strain of P pastoris KM71H was optimized. The encoding gene is located under the controlof the AOX promoter, which is induced by methanol whose concentration is instrumental for these strain types. Fermentation was upscaled in bioreactors employing various media and several methanol-feeding strategies. It was found that fed

Název v anglickém jazyce

Upscale of recombinant alpha-L-rhamnosidase production by Pichia pastoris Mut(S) strain

Popis výsledku anglicky

Pichia pastoris is currently one of the most preferred microorganisms for recombinant enzyme production due to its efficient expression system. The advantages include the production of high amounts of recombinant proteins containing the appropriate posttranslational modifications and easy cultivation conditions. alpha-L-Rhamnosidase is a biotechnologically important enzyme in food and pharmaceutical industry, used for example in debittering of citrus fruit juices, rhamnose pruning from naringin, or enhancement of wine aromas, creating a demand for the production of an active and stable enzyme. The production of recombinant alpha-L-rhamnosidase cloned in the Mut(S) strain of P pastoris KM71H was optimized. The encoding gene is located under the controlof the AOX promoter, which is induced by methanol whose concentration is instrumental for these strain types. Fermentation was upscaled in bioreactors employing various media and several methanol-feeding strategies. It was found that fed

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

OCT 2015

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000363861000001

EID výsledku v databázi Scopus

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