Francisella tularensis type B Delta dsbA mutant protects against type A strain and induces strong inflammatory cytokine and Th1-like antibody response in vivo

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00506543" target="_blank" >RIV/61388971:_____/15:00506543 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/15:43875429

Výsledek na webu

<a href="https://academic.oup.com/femspd/article/73/8/ftv058/2467550" target="_blank" >https://academic.oup.com/femspd/article/73/8/ftv058/2467550</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femspd/ftv058" target="_blank" >10.1093/femspd/ftv058</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Francisella tularensis type B Delta dsbA mutant protects against type A strain and induces strong inflammatory cytokine and Th1-like antibody response in vivo

Popis výsledku v původním jazyce

Francisella tularensis subspecies tularensis is a highly virulent intracellular bacterial pathogen, causing the disease tularemia. However, a safe and effective vaccine for routine application against F. tularensis has not yet been developed. We have recently constructed the deletion mutants for the DsbA homolog protein (Delta dsbA/FSC200) and a hypothetical protein IglH (Delta iglH/FSC200) in the type B F. tularensis subsp. holarctica FSC200 strain, which exerted different protection capacity against parental virulent strain. In this study, we further investigated the immunological correlates for these different levels of protection provided by Delta dsbA/FSC200 and Delta iglH/FSC200 mutants. Our results show that Delta dsbA/FSC200 mutant, but not Delta iglH/FSC200 mutant, induces an early innate inflammatory response leading to strong Th1-like antibody response. Furthermore, vaccination with Delta dsbA/FSC200 mutant, but not with Delta iglH/FSC200, elicited protection against the subsequent challenge with type A SCHU S4 strain in mice. An immunoproteomic approach was used to map a spectrum of antigens targeted by Th1-like specific antibodies, and more than 80 bacterial antigens, including novel ones, were identified. Comparison of tularemic antigens recognized by the Delta dsbA/FSC200 post-vaccination and the SCHU S4 post-challenge sera then revealed the existence of 22 novel SCHU S4 specific antibody clones.

Název v anglickém jazyce

Francisella tularensis type B Delta dsbA mutant protects against type A strain and induces strong inflammatory cytokine and Th1-like antibody response in vivo

Popis výsledku anglicky

Francisella tularensis subspecies tularensis is a highly virulent intracellular bacterial pathogen, causing the disease tularemia. However, a safe and effective vaccine for routine application against F. tularensis has not yet been developed. We have recently constructed the deletion mutants for the DsbA homolog protein (Delta dsbA/FSC200) and a hypothetical protein IglH (Delta iglH/FSC200) in the type B F. tularensis subsp. holarctica FSC200 strain, which exerted different protection capacity against parental virulent strain. In this study, we further investigated the immunological correlates for these different levels of protection provided by Delta dsbA/FSC200 and Delta iglH/FSC200 mutants. Our results show that Delta dsbA/FSC200 mutant, but not Delta iglH/FSC200 mutant, induces an early innate inflammatory response leading to strong Th1-like antibody response. Furthermore, vaccination with Delta dsbA/FSC200 mutant, but not with Delta iglH/FSC200, elicited protection against the subsequent challenge with type A SCHU S4 strain in mice. An immunoproteomic approach was used to map a spectrum of antigens targeted by Th1-like specific antibodies, and more than 80 bacterial antigens, including novel ones, were identified. Comparison of tularemic antigens recognized by the Delta dsbA/FSC200 post-vaccination and the SCHU S4 post-challenge sera then revealed the existence of 22 novel SCHU S4 specific antibody clones.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pathogens and Disease

ISSN

2049-632X

e-ISSN

—

Svazek periodika

73

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

ftv058

Kód UT WoS článku

000362978200003

EID výsledku v databázi Scopus

2-s2.0-85010004132