Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00460004" target="_blank" >RIV/61388971:_____/16:00460004 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/16:10332396

Výsledek na webu

<a href="http://dx.doi.org/10.1261/rna.054452.115" target="_blank" >http://dx.doi.org/10.1261/rna.054452.115</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1261/rna.054452.115" target="_blank" >10.1261/rna.054452.115</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast

Popis výsledku v původním jazyce

The molecular mechanism of stop codon recognition by the release factor eRF1 in complex with eRF3 has been described in great detail; however, our understanding of what determines the difference in termination efficiencies among various stop codon tetranucleotides and how near-cognate (nc) tRNAs recode stop codons during programmed readthrough in Saccharomyces cerevisiae is still poor. Here, we show that UGA-C as the only tetranucleotide of all four possible combinations dramatically exacerbated the readthrough phenotype of the stop codon recognition-deficient mutants in eRF1. Since the same is true also for UAA-C and UAG-C, we propose that the exceptionally high readthrough levels that all three stop codons display when followed by cytosine are partially caused by the compromised sampling ability of eRF1, which specifically senses cytosine at the +4 position.

Název v anglickém jazyce

Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast

Popis výsledku anglicky

The molecular mechanism of stop codon recognition by the release factor eRF1 in complex with eRF3 has been described in great detail; however, our understanding of what determines the difference in termination efficiencies among various stop codon tetranucleotides and how near-cognate (nc) tRNAs recode stop codons during programmed readthrough in Saccharomyces cerevisiae is still poor. Here, we show that UGA-C as the only tetranucleotide of all four possible combinations dramatically exacerbated the readthrough phenotype of the stop codon recognition-deficient mutants in eRF1. Since the same is true also for UAA-C and UAG-C, we propose that the exceptionally high readthrough levels that all three stop codons display when followed by cytosine are partially caused by the compromised sampling ability of eRF1, which specifically senses cytosine at the +4 position.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

R N A

ISSN

1355-8382

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

456-466

Kód UT WoS článku

000371365400013

EID výsledku v databázi Scopus

2-s2.0-84958719751