A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00464656" target="_blank" >RIV/61388971:_____/16:00464656 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/16:43891010

Výsledek na webu

<a href="http://dx.doi.org/10.1002/anie.201509920" target="_blank" >http://dx.doi.org/10.1002/anie.201509920</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/anie.201509920" target="_blank" >10.1002/anie.201509920</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway

Popis výsledku v původním jazyce

Cyanobactins are a rapidly growing family of linear and cyclic peptides produced by cyanobacteria. KawaguchipeptinsA and B, two macrocyclic undecapeptides reported earlier from Microcystis aeruginosa NIES-88, are shown to be products of the cyanobactin biosynthetic pathway. The 9kb kawaguchipeptin (kgp) gene cluster was identified in a 5.26Mb draft genome of Microcystis aeruginosa NIES-88. We verified that this gene cluster is responsible for the production of the kawaguchipeptins through heterologous expression of the kgp gene cluster in Escherichia coli. The KgpF prenyltransferase was overexpressed and was shown to prenylate C-3 of Trp residues in both linear and cyclic peptides invitro. Our findings serve to further enhance the structural diversity of cyanobactins to include tryptophan-prenylated cyclic peptides.

Název v anglickém jazyce

A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway

Popis výsledku anglicky

Cyanobactins are a rapidly growing family of linear and cyclic peptides produced by cyanobacteria. KawaguchipeptinsA and B, two macrocyclic undecapeptides reported earlier from Microcystis aeruginosa NIES-88, are shown to be products of the cyanobactin biosynthetic pathway. The 9kb kawaguchipeptin (kgp) gene cluster was identified in a 5.26Mb draft genome of Microcystis aeruginosa NIES-88. We verified that this gene cluster is responsible for the production of the kawaguchipeptins through heterologous expression of the kgp gene cluster in Escherichia coli. The KgpF prenyltransferase was overexpressed and was shown to prenylate C-3 of Trp residues in both linear and cyclic peptides invitro. Our findings serve to further enhance the structural diversity of cyanobactins to include tryptophan-prenylated cyclic peptides.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/LO1416" target="_blank" >LO1416: Algatech plus</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Angewandte Chemie - International Edition

ISSN

1433-7851

e-ISSN

—

Svazek periodika

55

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

4

Strana od-do

3596-3599

Kód UT WoS článku

000371521000007

EID výsledku v databázi Scopus

2-s2.0-84961147896