Seasonal variation and distribution of total and active microbial community of beta-glucosidase encoding genes in coniferous forest soil

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00474445" target="_blank" >RIV/61388971:_____/17:00474445 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.soilbio.2016.11.003" target="_blank" >http://dx.doi.org/10.1016/j.soilbio.2016.11.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.soilbio.2016.11.003" target="_blank" >10.1016/j.soilbio.2016.11.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Seasonal variation and distribution of total and active microbial community of beta-glucosidase encoding genes in coniferous forest soil

Popis výsledku v původním jazyce

Cellulose is the most abundant polysaccharide in the dead plant biomass, and its degradation is an important part of global carbon cycle. Beta-Glucosidases complete the final step of cellulose hydrolysis by converting cellobiose to glucose. Genetic potential and expression of Beta-glucosidase genes were studied in the topsoil of a Picea abies forest in two contrasting seasons. These seasons were the summer, representing the peak of plant photosynthetic activity, and late winter, after an extended period with no photosynthate input. Fungal and bacterial beta-glucosidase genes belonging to glycoside hydrolase families GH1 and GH3 amplified from DNA and RNA and amplicon pools were analyzed. Transcript pool were largely corresponded to gene pools, although some abundant transcripts were not found in the gene pool. The major reservoirs of Beta-glucosidase genes were the fungal phylum Ascomycota and Basidiomycota and the bacterial phyla Firmicutes, Actinobacteria, Proteobacteria Acidobacteria and Deinococcus-Thermus. This indicates that a diverse microbial community utilized cellobiose. Seasonality influenced both genetic diversity of Beta-glucosidase genes and their expression. The results indicate that a complex community of bacteria and fungi expresses beta-glucosidases in forest soils. Even Beta-glucosidase genes showing low abundance in DNA may be functionally important as revealed by their high expression especially in basidiomycota. The functional diversity in the studied ecosystem clearly exhibited a seasonal pattern.

Název v anglickém jazyce

Seasonal variation and distribution of total and active microbial community of beta-glucosidase encoding genes in coniferous forest soil

Popis výsledku anglicky

Cellulose is the most abundant polysaccharide in the dead plant biomass, and its degradation is an important part of global carbon cycle. Beta-Glucosidases complete the final step of cellulose hydrolysis by converting cellobiose to glucose. Genetic potential and expression of Beta-glucosidase genes were studied in the topsoil of a Picea abies forest in two contrasting seasons. These seasons were the summer, representing the peak of plant photosynthetic activity, and late winter, after an extended period with no photosynthate input. Fungal and bacterial beta-glucosidase genes belonging to glycoside hydrolase families GH1 and GH3 amplified from DNA and RNA and amplicon pools were analyzed. Transcript pool were largely corresponded to gene pools, although some abundant transcripts were not found in the gene pool. The major reservoirs of Beta-glucosidase genes were the fungal phylum Ascomycota and Basidiomycota and the bacterial phyla Firmicutes, Actinobacteria, Proteobacteria Acidobacteria and Deinococcus-Thermus. This indicates that a diverse microbial community utilized cellobiose. Seasonality influenced both genetic diversity of Beta-glucosidase genes and their expression. The results indicate that a complex community of bacteria and fungi expresses beta-glucosidases in forest soils. Even Beta-glucosidase genes showing low abundance in DNA may be functionally important as revealed by their high expression especially in basidiomycota. The functional diversity in the studied ecosystem clearly exhibited a seasonal pattern.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA16-08916S" target="_blank" >GA16-08916S: Faktory ovlivňující strukturu a funkci společenstev hub v ekosystému jehličnatého lesa</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Soil Biology and Biochemistry

ISSN

0038-0717

e-ISSN

—

Svazek periodika

105

Číslo periodika v rámci svazku

February

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

71-80

Kód UT WoS článku

000392788600010

EID výsledku v databázi Scopus

2-s2.0-84997293631